Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression

Clark, Lindsay V.; Stewart, J. Ryan; Nishiwaki, Aya; Toma, Yo; Kjeldsen, Jens Bonderup; Jorgensen, Uffe; Zhao, Hua; Peng, Junhua; Yoo, Ji Hye; Heo, Kweon; Yu, Chang Yeon; Yamada, Toshihiko; Sacks, Erik J.

doi:10.1093/jxb/eru511

Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression

Journal Article · Sat Jan 24 00:00:00 EST 2015 · Journal of Experimental Botany

DOI:https://doi.org/10.1093/jxb/eru511· OSTI ID:1344512

Clark, Lindsay V. ^[1]; Stewart, J. Ryan ^[2]; Nishiwaki, Aya ^[3]; Toma, Yo ^[4]; Kjeldsen, Jens Bonderup ^[5]; Jorgensen, Uffe ^[5]; Zhao, Hua ^[6]; Peng, Junhua ^[7]; Yoo, Ji Hye ^[8]; Heo, Kweon ^[8]; Yu, Chang Yeon ^[8]; Yamada, Toshihiko ^[9]; Sacks, Erik J. ^[10]

Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States); DOE Office of Scientific and Technical Information (OSTI)
Brigham Young Univ., Provo, UT (United States)
Univ. of Miyazaki, Miyazaki (Japan)
Ehime Univ., Ehime (Japan)
Aarhus Univ. (Denmark)
Huazhong Agricultural Univ., Hubei (China)
China Seed Group Co. Ltd., Hubei (China)
Kangwon National Univ., Gangwon (South Korea)
Hokkaido Univ., Hokkaido (Japan)
Univ. of Illinois, Urbana-Champaign, Urbana, IL (United States)

Unilateral introgression from diploids to tetraploids has been hypothesized to be an important evolutionary mechanism in plants. However, few examples have been definitively identified, perhaps because data of sufficient depth and breadth were difficult to obtain before the advent of affordable high-density genotyping. Throughout Japan, tetraploid Miscanthus sacchariflorus and diploid Miscanthus sinensis are common, and occasionally hybridize. In this study, 667 M. sinensis and 78 M. sacchariflorus genotypes from Japan were characterized using 20 704 SNPs and ten plastid microsatellites. Similarity of SNP genotypes between diploid and tetraploid M. sacchariflorus indicated that the tetraploids originated through autopolyploidy. Structure analysis indicated a gradient of introgression from diploid M. sinensis into tetraploid M. sacchariflorus throughout Japan; most tetraploids had some M. sinensis DNA. Among phenotypically M. sacchariflorus tetraploids, M. sinensis ancestry averaged 7% and ranged from 1-39%, with introgression greatest in southern Japan. Unexpectedly, rare (~1%) diploid M. sinensis individuals from northern Japan were found with 6-27% M. sacchariflorus ancestry. Population structure of M. sinensis in Japan included three groups, and was driven primarily by distance, and secondarily by geographic barriers such as mountains and straits. Miscanthus speciation is a complex and dynamic process. In contrast to limited introgression between diploid M. sacchariflorus and M. sinensis in northern China, selection for adaptation to a moderate maritime climate probably favoured cross-ploidy introgressants in southern Japan. Ultimately, these results will help guide the selection of Miscanthus accessions for the breeding of biomass cultivars.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Illinois, Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0012379

OSTI ID:: 1344512

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 14 Vol. 66; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Genetic structure of Miscanthus sinensis and Miscanthus sacchariflorus in Japan indicates a gradient of bidirectional but asymmetric introgression

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