Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants

Aung, Kyaw; Xin, Xiufang; Mecey, Christy; He, Sheng Yang

doi:10.1007/978-1-4939-6649-3_12

Title: Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants

Journal Article · Sat Nov 12 00:00:00 EST 2016 · Methods in Molecular Biology (Online)

DOI:https://doi.org/10.1007/978-1-4939-6649-3_12· OSTI ID:1607990

Aung, Kyaw ^[1]; Xin, Xiufang ^[2]; Mecey, Christy ^[2]; He, Sheng Yang ^[1]

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory and Howard Hughes Medical Inst.
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector system is a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors, which target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular bases of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.

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Research Organization:: Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1607990

Journal Information:: Methods in Molecular Biology (Online), Vol. 1531; Related Information: Chapter 12; ISSN 1940-6029

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 11 works

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References (19)

Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean. Whalen, M. C.; Innes, R. W.; Bent, A. F. The Plant Cell, Vol. 3, Issue 1 https://doi.org/10.1105/tpc.3.1.49	journal	January 1991
Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set Geldner, Niko; Dénervaud-Tendon, Valérie; Hyman, Derek L. The Plant Journal, Vol. 59, Issue 1 https://doi.org/10.1111/j.1365-313X.2009.03851.x	journal	July 2009
Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis Nomura, K.; Mecey, C.; Lee, Y. -N. Proceedings of the National Academy of Sciences, Vol. 108, Issue 26 https://doi.org/10.1073/pnas.1103338108	journal	June 2011
T-DNA Binary Vectors and Systems Lee, Lan-Ying; Gelvin, Stanton B. Plant Physiology, Vol. 146, Issue 2 https://doi.org/10.1104/pp.107.113001	journal	February 2008
A guide to choosing fluorescent proteins Shaner, Nathan C.; Steinbach, Paul A.; Tsien, Roger Y. Nature Methods, Vol. 2, Issue 12 https://doi.org/10.1038/nmeth819	journal	November 2005
In-Depth Proteome Analysis of Arabidopsis Leaf Peroxisomes Combined with in Vivo Subcellular Targeting Verification Indicates Novel Metabolic and Regulatory Functions of Peroxisomes Reumann, Sigrun; Quan, Sheng; Aung, Kyaw Plant Physiology, Vol. 150, Issue 1 https://doi.org/10.1104/pp.109.137703	journal	March 2009
Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation Nakagawa, Tsuyoshi; Kurose, Takayuki; Hino, Takeshi Journal of Bioscience and Bioengineering, Vol. 104, Issue 1 https://doi.org/10.1263/jbb.104.34	journal	July 2007
A type III effector ADP-ribosylates RNA-binding proteins and quells plant immunity Fu, Zheng Qing; Guo, Ming; Jeong, Byeong-ryool Nature, Vol. 447, Issue 7142 https://doi.org/10.1038/nature05737	journal	April 2007
Gateway-compatible vectors for plant functional genomics and proteomics Earley, Keith W.; Haag, Jeremy R.; Pontes, Olga The Plant Journal, Vol. 45, Issue 4 https://doi.org/10.1111/j.1365-313X.2005.02617.x	journal	February 2006
The Pseudomonas syringae type III effector HopD1 suppresses effector-triggered immunity, localizes to the endoplasmic reticulum, and targets the Arabidopsis transcription factor NTL9 Block, Anna; Toruño, Tania Y.; Elowsky, Christian G. New Phytologist, Vol. 201, Issue 4 https://doi.org/10.1111/nph.12626	journal	December 2013
A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses: P. syringae HopN1 targets photosynthesis Rodríguez-Herva, José J.; González-Melendi, Pablo; Cuartas-Lanza, Raquel Cellular Microbiology, Vol. 14, Issue 5 https://doi.org/10.1111/j.1462-5822.2012.01749.x	journal	February 2012
A J Domain Virulence Effector of Pseudomonas syringae Remodels Host Chloroplasts and Suppresses Defenses Jelenska, Joanna; Yao, Nan; Vinatzer, Boris A. Current Biology, Vol. 17, Issue 6 https://doi.org/10.1016/j.cub.2007.02.028	journal	March 2007
A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants: Fluorescent organelle markers Nelson, Brook K.; Cai, Xue; Nebenführ, Andreas The Plant Journal, Vol. 51, Issue 6 https://doi.org/10.1111/j.1365-313X.2007.03212.x	journal	August 2007
Phosphorylation of HopQ1, a Type III Effector from Pseudomonas syringae , Creates a Binding Site for Host 14-3-3 Proteins Giska, Fabian; Lichocka, Małgorzata; Piechocki, Marcin Plant Physiology, Vol. 161, Issue 4 https://doi.org/10.1104/pp.112.209023	journal	February 2013
Distinct Pseudomonas type-III effectors use a cleavable transit peptide to target chloroplasts Li, Guangyong; Froehlich, John E.; Elowsky, Christian The Plant Journal, Vol. 77, Issue 2 https://doi.org/10.1111/tpj.12396	journal	December 2013
The Pseudomonas syringae type III effector HopG1 targets mitochondria, alters plant development and suppresses plant innate immunity Block, Anna; Guo, Ming; Li, Guangyong Cellular Microbiology, Vol. 12, Issue 3 https://doi.org/10.1111/j.1462-5822.2009.01396.x	journal	March 2010
Pseudomonas syringae pv. tomato DC3000 Type III Secretion Effector Polymutants Reveal an Interplay between HopAD1 and AvrPtoB Wei, Hai-Lei; Chakravarthy, Suma; Mathieu, Johannes Cell Host & Microbe, Vol. 17, Issue 6 https://doi.org/10.1016/j.chom.2015.05.007	journal	June 2015
Avoiding the Ends: Internal Epitope Tagging of Proteins Using Transposon Tn7 Zordan, R. E.; Beliveau, B. J.; Trow, J. A. Genetics, Vol. 200, Issue 1 https://doi.org/10.1534/genetics.114.169482	journal	March 2015
Pseudomonas syringae Effector Avirulence Protein E Localizes to the Host Plasma Membrane and Down-Regulates the Expression of the NONRACE-SPECIFIC DISEASE RESISTANCE1/HARPIN-INDUCED1-LIKE13 Gene Required for Antibacterial Immunity in Arabidopsis Xin, Xiu-Fang; Nomura, Kinya; Ding, Xinhua Plant Physiology, Vol. 169, Issue 1 https://doi.org/10.1104/pp.15.00547	journal	July 2015

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59 BASIC BIOLOGICAL SCIENCES
Plant pathogen
Bacterial pathogenesis
Type III secretion
Plant immunity
Tobacco
Arabidopsis thaliana
Agrobacterium
Confocal microscopy

Title: Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants

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