Characterization of a nitrogen-regulated protein identified by cell surface biotinylation of a marine phytoplankton

Journal Article · Fri Sep 01 04:00:00 EDT 1995 · Applied and Environmental Microbiology

OSTI ID:159903

Palenik, B; Koke, J A ^[1]

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1. Univ. of California, San Diego, La Jolla, CA (United States)

The biotinylating reagent succinimidyl 6-(biotinamido) hexanoate was used to label the cell surfaces of the cosmopolitan, marine, eukaryotic microorganism Emiliania huxleyi under different growth conditions. Proteins characteristic of different nutrient conditions could be identified. In particular, a nitrogen-regulated protein, nrp1, has an 82-kDa subunit that is present under nitrogen limitation and during growth on urea. It is absent under phosphate limitation or during exponential growth on nitrate or ammonia. nrp1 is the major membrane or wall protein in nitrogen-limited cells and is found in several strains of E. huxleyi. It may be a useful biomarker for examining the physiological state of E. huxleyi cells in their environment. 35 refs., 4 figs.

• DOE Contract Number: FG03-93ER61692
• OSTI ID: 159903
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 9 Vol. 61; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOCHEMISTRY
ENZYMES
EVALUATION
PHYTOPLANKTON
SURFACES