Mutation of Mediator subunit $CDK$ counteracts the stunted growth and salicylic acid hyperaccumulation phenotypes of an Arabidopsis $MED$ mutant

Mao, Xiangying; Kim, Jeong Im; Wheeler, Mitchell T.; Heintzelman, Anne K.; Weake, Vikki M.; Chapple, Clint

doi:10.1111/nph.15741

Mutation of Mediator subunit $CDK$ counteracts the stunted growth and salicylic acid hyperaccumulation phenotypes of an Arabidopsis $MED$ mutant

Journal Article · Mon Mar 18 00:00:00 EDT 2019 · New Phytologist

DOI:https://doi.org/10.1111/nph.15741· OSTI ID:1597908

^[1]; ^[2]; Wheeler, Mitchell T. ^[2]; Heintzelman, Anne K. ^[3]; ^[4]; ^[5]

Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry; Purdue Univ., West Lafayette, IN (United States). Purdue Center for Plant Biology; Purdue University
Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry
Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry; Northwest Missouri State Univ., Maryville, MO (United States)
Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry; Purdue Univ., West Lafayette, IN (United States). Purdue University Center for Cancer Research
Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry; Purdue Univ., West Lafayette, IN (United States). Purdue Center for Plant Biology

The Mediator complex functions as a hub for transcriptional regulation. MED5, an Arabidopsis Mediator tail subunit, is required for maintaining phenylpropanoid homeostasis. A semidominant mutation (ref4-3) that causes a single amino acid substitution in MED5b functions as a strong suppressor of the pathway, leading to decreased soluble phenylpropanoid accumulation, reduced lignin content and dwarfism. By contrast, loss of MED5 results in increased concentrations of phenylpropanoids. We used a reverse genetic approach to identify suppressors of ref4-3 and found that ref4-3 requires CDK8, a kinase module subunit of Mediator, to repress plant growth. The genetic interaction between MED5 and CDK8 was further characterized using mRNA-sequencing (RNA-seq) and metabolite analysis. Growth inhibition and suppression of phenylpropanoid metabolism can be genetically separated in ref4-3 by elimination of CDK8 kinase activity; however, the stunted growth of ref4-3 is not dependent on the phosphorylation event introduced by the G383S mutation. In addition, rather than perturbation of lignin biosynthesis, misregulation of DJC66, a gene encoding a DNAJ protein, is involved in the dwarfism of the med5 mutants. Together, our study reveals genetic interactions between Mediator tail and kinase module subunits and enhances our understanding of dwarfing in phenylpropanoid pathway mutants.

View Accepted Manuscript (DOE)

Research Organization:: Purdue Univ., West Lafayette, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-07ER15905

OSTI ID:: 1597908

Alternate ID(s):: OSTI ID: 1501715

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 1 Vol. 223; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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