Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Xia, Mingqian; Fu, Dafang; Chakraborty, Romy; Singh, Rajendra Prasad; Terry, Norman

doi:10.1016/j.biortech.2019.01.131

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

Journal Article · Tue Jan 29 00:00:00 EST 2019 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2019.01.131· OSTI ID:1580839

Xia, Mingqian ^[1]; Fu, Dafang ^[2]; Chakraborty, Romy ^[3]; Singh, Rajendra Prasad ^[2]; Terry, Norman ^[4]

Southeast Univ., Nanjing (China); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Southeast Univ., Nanjing (China)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States)

The aim of this work was to study the production of bioemulsifier by Rhodococcus erythropolis OSDS1, and the improvement of crude oil depletion efficiency using a consortium of petroleum hydrocarbon degraders and OSDS1. The results showed that R. erythropolis OSDS1 produced highly stable bioemulsifier under various salinity (0-35 g/L NaCl) and pH (5.0-9.0) conditions; more than 90% of the initial emulsification activity was retained after 168 h. Emulsification capacity of the bioemulsifier on different petroleum hydrocarbons was diesel > mineral oil/crude oil > gasoline. A mixed bacterial consortium combining OSDS1 and four other petroleum hydrocarbon degraders was constructed. GC-MS results revealed that the constructed consortium achieved 85.26% depletion efficiency of crude oil in 15 days, which was significantly higher than that of individual strains. During the process, we observed alkane hydroxylase gene (alkB) was successfully amplified from the consortium, confirming presence of crude oil degrading enzymes.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: China Scholarship Council (CSC); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1580839

Alternate ID(s):: OSTI ID: 2395995

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 282; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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02 PETROLEUM
54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
Rhodococcus erythropolis
alkB gene
bioemulsifier
constructed bacterial consortium
crude oil depletion

Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders

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