Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts

Rodriguez, Alberto; Ersig, Nadine; Geiselman, Gina M.; Seibel, Katharina; Simmons, Blake A.; Magnuson, Jon K.; Eudes, Aymerick; Gladden, John M.

doi:10.1016/j.biortech.2019.121365

Title: Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts

Journal Article · Thu Aug 01 00:00:00 EDT 2019 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2019.121365· OSTI ID:1572180

Rodriguez, Alberto ^[1]; Ersig, Nadine ^[2]; Geiselman, Gina M. ^[1]; Seibel, Katharina ^[2]; Simmons, Blake A. ^[3];

^[4]; Eudes, Aymerick ^[3]; Gladden, John M. ^[5]

Sandia National Laboratory
Mannheim University of Applied Sciences
Lawrence Berkeley National Laboratory
BATTELLE (PACIFIC NW LAB)
SANDIA NATIONAL LAB

One of the requirements for efficient biological conversion of lignocellulose to bioproducts is the compatibility of biological catalysts with the processes employed to solubilize and depolymerize the lignocellulosic components. The red yeasts Rhodosporidium toruloides and Rhodotorula mucilaginosa were evaluated for their ability to assimilate sugars and aromatic compounds extracted from two engineered lines of Arabidopsis thaliana with modified lignin or the wild-type using ionic liquid, acid or alkaline pretreatments. Differential amounts of monomeric sugars, organic acids and, in the case of the engineered lines, either 4-hydroxybenzoic or protocatechuic acid were additionally released from the biomass and found to be tolerated and consumed by both microorganisms. Genetically-engineered strains of the two red yeasts successfully converted the depolymerized products into the biofuel precursor bisabolene when cultivated on hydrolysates or synthetic media containing specific sugars, acids and aromatics found in the hydrolysates.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1572180

Report Number(s):: PNNL-SA-148133

Journal Information:: Bioresource Technology, Vol. 286

Country of Publication:: United States

Language:: English

References (38)

Biological valorization of low molecular weight lignin Abdelaziz, Omar Y.; Brink, Daniel P.; Prothmann, Jens Biotechnology Advances, Vol. 34, Issue 8 https://doi.org/10.1016/j.biotechadv.2016.10.001	journal	December 2016
A history of research on yeasts 13. Active transport and the uptake of various metabolites ¹ Barnett, James A. Yeast, Vol. 25, Issue 10 https://doi.org/10.1002/yea.1630	journal	October 2008
Opportunities and challenges in biological lignin valorization Beckham, Gregg T.; Johnson, Christopher W.; Karp, Eric M. Current Opinion in Biotechnology, Vol. 42 https://doi.org/10.1016/j.copbio.2016.02.030	journal	December 2016
Importance of chemical pretreatment for bioconversion of lignocellulosic biomass Behera, Shuvashish; Arora, Richa; Nandhagopal, N. Renewable and Sustainable Energy Reviews, Vol. 36 https://doi.org/10.1016/j.rser.2014.04.047	journal	August 2014
Insight into progress in pre-treatment of lignocellulosic biomass Bhutto, Abdul Waheed; Qureshi, Khadija; Harijan, Khanji Energy, Vol. 122 https://doi.org/10.1016/j.energy.2017.01.005	journal	March 2017
Lignocellulose deconstruction in the biosphere Bomble, Yannick J.; Lin, Chien-Yuan; Amore, Antonella Current Opinion in Chemical Biology, Vol. 41 https://doi.org/10.1016/j.cbpa.2017.10.013	journal	December 2017
Enzymatic conversion of lignin into renewable chemicals Bugg, Timothy DH; Rahmanpour, Rahman Current Opinion in Chemical Biology, Vol. 29 https://doi.org/10.1016/j.cbpa.2015.06.009	journal	December 2015
Biosynthesis and incorporation of side-chain-truncated lignin monomers to reduce lignin polymerization and enhance saccharification: Reduction in lignin DP enhances saccharification Eudes, Aymerick; George, Anthe; Mukerjee, Purba Plant Biotechnology Journal, Vol. 10, Issue 5 https://doi.org/10.1111/j.1467-7652.2012.00692.x	journal	March 2012
Expression of a bacterial 3-dehydroshikimate dehydratase reduces lignin content and improves biomass saccharification efficiency Eudes, Aymerick; Sathitsuksanoh, Noppadon; Baidoo, Edward E. K. Plant Biotechnology Journal, Vol. 13, Issue 9 https://doi.org/10.1111/pbi.12310	journal	January 2015
Pretreatments to enhance the digestibility of lignocellulosic biomass Hendriks, A. T. W. M.; Zeeman, G. Bioresource Technology, Vol. 100, Issue 1 https://doi.org/10.1016/j.biortech.2008.05.027	journal	January 2009
Pretreatment and Lignocellulosic Chemistry Hu, Fan; Ragauskas, Art BioEnergy Research, Vol. 5, Issue 4 https://doi.org/10.1007/s12155-012-9208-0	journal	May 2012
Bioconversion of lignocellulose: inhibitors and detoxification Jönsson, Leif J.; Alriksson, Björn; Nilvebrant, Nils-Olof Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-16	journal	January 2013
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects Jönsson, Leif J.; Martín, Carlos Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.10.009	journal	January 2016
Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass Klinke, H. B.; Thomsen, A. B.; Ahring, B. K. Applied Microbiology and Biotechnology, Vol. 66, Issue 1, p. 10-26 https://doi.org/10.1007/s00253-004-1642-2	journal	August 2004
Compounds inhibiting the bioconversion of hydrothermally pretreated lignocellulose Ko, Ja Kyong; Um, Youngsoon; Park, Yong-Cheol Applied Microbiology and Biotechnology, Vol. 99, Issue 10 https://doi.org/10.1007/s00253-015-6595-0	journal	April 2015
Lignin valorization through integrated biological funneling and chemical catalysis Linger, J. G.; Vardon, D. R.; Guarnieri, M. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018 https://doi.org/10.1073/pnas.1410657111	journal	August 2014
Impact of Dilute Sulfuric Acid, Ammonium Hydroxide, and Ionic Liquid Pretreatments on the Fractionation and Characterization of Engineered Switchgrass Liu, Enshi; Das, Lalitendu; Zhao, Bingyu BioEnergy Research, Vol. 10, Issue 4 https://doi.org/10.1007/s12155-017-9868-x	journal	August 2017
Natural deep eutectic solvents (DES) for fractionation of waste lignocellulosic biomass and its cascade conversion to value-added bio-based chemicals Mamilla, Jhansi L. K.; Novak, Uroš; Grilc, Miha Biomass and Bioenergy, Vol. 120 https://doi.org/10.1016/j.biombioe.2018.12.002	journal	January 2019
Trends in bioconversion of lignocellulose: Biofuels, platform chemicals & biorefinery concept Menon, Vishnu; Rao, Mala Progress in Energy and Combustion Science, Vol. 38, Issue 4 https://doi.org/10.1016/j.pecs.2012.02.002	journal	August 2012
Engineering Sugar Utilization and Microbial Tolerance toward Lignocellulose Conversion Nieves, Lizbeth M.; Panyon, Larry A.; Wang, Xuan Frontiers in Bioengineering and Biotechnology, Vol. 3 https://doi.org/10.3389/fbioe.2015.00017	journal	February 2015
Microbial utilization of lignin: available biotechnologies for its degradation and valorization Palazzolo, Martín A.; Kurina-Sanz, Marcela World Journal of Microbiology and Biotechnology, Vol. 32, Issue 10 https://doi.org/10.1007/s11274-016-2128-y	journal	August 2016
Sustainable biodiesel production from oleaginous yeasts utilizing hydrolysates of various non-edible lignocellulosic biomasses Patel, Alok; Arora, Neha; Sartaj, Km Renewable and Sustainable Energy Reviews, Vol. 62 https://doi.org/10.1016/j.rser.2016.05.014	journal	September 2016
Identification and microbial production of a terpene-based advanced biofuel Peralta-Yahya, Pamela P.; Ouellet, Mario; Chan, Rossana Nature Communications, Vol. 2, Issue 1 https://doi.org/10.1038/ncomms1494	journal	September 2011
Efficient oleaginous yeasts for lipid production from lignocellulosic sugars and effects of lignocellulose degradation compounds on growth and lipid production Poontawee, Rujiralai; Yongmanitchai, Wichien; Limtong, Savitree Process Biochemistry, Vol. 53 https://doi.org/10.1016/j.procbio.2016.11.013	journal	February 2017
Various pretreatments of lignocellulosics Rabemanolontsoa, Harifara; Saka, Shiro Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.08.029	journal	January 2016
Lignin Valorization: Improving Lignin Processing in the Biorefinery Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J. Science, Vol. 344, Issue 6185, p. 1246843-1246843 https://doi.org/10.1126/science.1246843	journal	May 2014
Hydroxycinnamates in lignification Ralph, John Phytochemistry Reviews, Vol. 9, Issue 1, p. 65-83 https://doi.org/10.1007/s11101-009-9141-9	journal	August 2009
Paving the Way for Lignin Valorisation: Recent Advances in Bioengineering, Biorefining and Catalysis Rinaldi, Roberto; Jastrzebski, Robin; Clough, Matthew T. Angewandte Chemie International Edition, Vol. 55, Issue 29 https://doi.org/10.1002/anie.201510351	journal	June 2016
Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion Rodriguez, Alberto; Salvachúa, Davinia; Katahira, Rui ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 9 https://doi.org/10.1021/acssuschemeng.7b01818	journal	August 2017
Metabolic Engineering of Oleaginous Yeasts for Production of Fuels and Chemicals Shi, Shuobo; Zhao, Huimin Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.02185	journal	November 2017
Biological pretreatment of lignocellulosic biomass – An overview Sindhu, Raveendran; Binod, Parameswaran; Pandey, Ashok Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.08.030	journal	January 2016
Demonstrating a separation-free process coupling ionic liquid pretreatment, saccharification, and fermentation with Rhodosporidium toruloides to produce advanced biofuels Sundstrom, Eric; Yaegashi, Junko; Yan, Jipeng Green Chemistry, Vol. 20, Issue 12 https://doi.org/10.1039/C8GC00518D	journal	January 2018
Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products Xu, Zhaoxian; Lei, Peng; Zhai, Rui Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1376-0	journal	February 2019
Rhodosporidium toruloides: a new platform organism for conversion of lignocellulose into terpene biofuels and bioproducts Yaegashi, Junko; Kirby, James; Ito, Masakazu Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0927-5	journal	October 2017
Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid Yu, Xiaochen; Zheng, Yubin; Dorgan, Kathleen M. Bioresource Technology, Vol. 102, Issue 10 https://doi.org/10.1016/j.biortech.2011.02.081	journal	May 2011
Effect of selected aldehydes on the growth and fermentation of ethanologenic Escherichia coli Zaldivar, Jesus; Martinez, Alfredo; Ingram, Lonnie O. Biotechnology and Bioengineering, Vol. 65, Issue 1, p. 24-33 https://doi.org/10.1002/(SICI)1097-0290(19991005)65:1<24::AID-BIT4>3.0.CO;2-2	journal	October 1999
Organic solvent pretreatment of lignocellulosic biomass for biofuels and biochemicals: A review Zhang, Ke; Pei, Zhijian; Wang, Donghai Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.08.102	journal	January 2016
Engineering Rhodosporidium toruloides for increased lipid production : Engineering Zhang, Shuyan; Skerker, Jeffrey M.; Rutter, Charles D. Biotechnology and Bioengineering, Vol. 113, Issue 5 https://doi.org/10.1002/bit.25864	journal	November 2015

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Production of ent-kaurene from lignocellulosic hydrolysate in Rhodosporidium toruloides Geiselman, Gina M.; Zhuang, Xun; Kirby, James Microbial Cell Factories, Vol. 19, Issue 1 https://doi.org/10.1186/s12934-020-1293-8	journal	February 2020

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