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Title: Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts

Abstract

One of the requirements for efficient biological conversion of lignocellulose to bioproducts is the compatibility of biological catalysts with the processes employed to solubilize and depolymerize the lignocellulosic components. The red yeasts Rhodosporidium toruloides and Rhodotorula mucilaginosa were evaluated for their ability to assimilate sugars and aromatic compounds extracted from two engineered lines of Arabidopsis thaliana with modified lignin or the wild-type using ionic liquid, acid or alkaline pretreatments. Differential amounts of monomeric sugars, organic acids and, in the case of the engineered lines, either 4-hydroxybenzoic or protocatechuic acid were additionally released from the biomass and found to be tolerated and consumed by both microorganisms. Genetically-engineered strains of the two red yeasts successfully converted the depolymerized products into the biofuel precursor bisabolene when cultivated on hydrolysates or synthetic media containing specific sugars, acids and aromatics found in the hydrolysates.

Authors:
 [1];  [2];  [1];  [2];  [3]; ORCiD logo [4];  [3];  [5]
  1. Sandia National Laboratory
  2. Mannheim University of Applied Sciences
  3. Lawrence Berkeley National Laboratory
  4. BATTELLE (PACIFIC NW LAB)
  5. SANDIA NATIONAL LAB
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1572180
Report Number(s):
PNNL-SA-148133
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 286
Country of Publication:
United States
Language:
English

Citation Formats

Rodriguez, Alberto, Ersig, Nadine, Geiselman, Gina M., Seibel, Katharina, Simmons, Blake A., Magnuson, Jon K., Eudes, Aymerick, and Gladden, John M. Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts. United States: N. p., 2019. Web. doi:10.1016/j.biortech.2019.121365.
Rodriguez, Alberto, Ersig, Nadine, Geiselman, Gina M., Seibel, Katharina, Simmons, Blake A., Magnuson, Jon K., Eudes, Aymerick, & Gladden, John M. Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts. United States. doi:10.1016/j.biortech.2019.121365.
Rodriguez, Alberto, Ersig, Nadine, Geiselman, Gina M., Seibel, Katharina, Simmons, Blake A., Magnuson, Jon K., Eudes, Aymerick, and Gladden, John M. Thu . "Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts". United States. doi:10.1016/j.biortech.2019.121365.
@article{osti_1572180,
title = {Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeasts},
author = {Rodriguez, Alberto and Ersig, Nadine and Geiselman, Gina M. and Seibel, Katharina and Simmons, Blake A. and Magnuson, Jon K. and Eudes, Aymerick and Gladden, John M.},
abstractNote = {One of the requirements for efficient biological conversion of lignocellulose to bioproducts is the compatibility of biological catalysts with the processes employed to solubilize and depolymerize the lignocellulosic components. The red yeasts Rhodosporidium toruloides and Rhodotorula mucilaginosa were evaluated for their ability to assimilate sugars and aromatic compounds extracted from two engineered lines of Arabidopsis thaliana with modified lignin or the wild-type using ionic liquid, acid or alkaline pretreatments. Differential amounts of monomeric sugars, organic acids and, in the case of the engineered lines, either 4-hydroxybenzoic or protocatechuic acid were additionally released from the biomass and found to be tolerated and consumed by both microorganisms. Genetically-engineered strains of the two red yeasts successfully converted the depolymerized products into the biofuel precursor bisabolene when cultivated on hydrolysates or synthetic media containing specific sugars, acids and aromatics found in the hydrolysates.},
doi = {10.1016/j.biortech.2019.121365},
journal = {Bioresource Technology},
number = ,
volume = 286,
place = {United States},
year = {2019},
month = {8}
}