Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts

Lin, Chien-Yuan; Geiselman, Gina M.; Liu, Di; Magurudeniya, Harsha D.; Rodriguez, Alberto; Chen, Yi-Chun; Pidatala, Venkataramana; Unda, Faride; Amer, Bashar; Baidoo, Edward E. K.; Mansfield, Shawn D.; Simmons, Blake A.; Singh, Seema; Scheller, Henrik V.; Gladden, John M.; Eudes, Aymerick

doi:10.1186/s13068-022-02245-4

Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts

Journal Article · Sat Dec 24 23:00:00 EST 2022 · Biotechnology for Biofuels and Bioproducts

DOI:https://doi.org/10.1186/s13068-022-02245-4· OSTI ID:1958752

Lin, Chien-Yuan ^[1]; Geiselman, Gina M. ^[2]; Liu, Di ^[3]; Magurudeniya, Harsha D. ^[4]; Rodriguez, Alberto ^[2]; Chen, Yi-Chun ^[1]; Pidatala, Venkataramana ^[1]; Unda, Faride ^[5]; Amer, Bashar ^[1]; Baidoo, Edward E. K. ^[1]; Mansfield, Shawn D. ^[6]; Simmons, Blake A. ^[1]; Singh, Seema ^[4]; Scheller, Henrik V. ^[7]; Gladden, John M. ^[2]; Eudes, Aymerick ^[1]

Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States); USDOE Agile BioFoundry, Emeryville, CA (United States)
Sandia National Lab. (SNL-CA), Livermore, CA (United States); USDOE Agile BioFoundry, Emeryville, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Univ. of British Columbia, Vancouver, BC (Canada)
Univ. of British Columbia, Vancouver, BC (Canada); Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)

Lignocellulosic resources are promising feedstocks for the manufacture of bio-based products and bioenergy. However, the inherent recalcitrance of biomass to conversion into simple sugars currently hinders the deployment of advanced bioproducts at large scale. Lignin is a primary contributor to biomass recalcitrance as it protects cell wall polysaccharides from degradation and can inhibit hydrolytic enzymes via non-productive adsorption. Several engineering strategies have been designed to reduce lignin or modify its monomeric composition. For example, expression of bacterial 3-dehydroshikimate dehydratase (QsuB) in poplar trees resulted in a reduction in lignin due to redirection of metabolic flux toward 3,4-dihydroxybenzoate at the expense of lignin. This reduction was accompanied with remarkable changes in the pools of aromatic compounds that accumulate in the biomass. The impact of these modifications on downstream biomass deconstruction and conversion into advanced bioproducts was evaluated in the current study. Using ionic liquid pretreatment followed by enzymatic saccharification, biomass from engineered trees released more glucose and xylose compared to wild-type control trees under optimum conditions. Fermentation of the resulting hydrolysates using Rhodosporidium toruloides strains engineered to produce α-bisabolene, epi-isozizaene, and fatty alcohols showed no negative impact on cell growth and yielded higher titers of bioproducts (as much as + 58%) in the case of QsuB transgenics trees. Our data show that low-recalcitrant poplar biomass obtained with the QsuB technology has the potential to improve the production of advanced bioproducts.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1958752

Journal Information:: Biotechnology for Biofuels and Bioproducts, Journal Name: Biotechnology for Biofuels and Bioproducts Journal Issue: 1 Vol. 15; ISSN 2731-3654

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates Sasaki, Yusuke; Eng, Thomas; Herbert, Robin Figshare https://doi.org/10.6084/m9.figshare.c.4417520.v1	collection	January 2019
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Conversion of poplar biomass into high-energy density tricyclic sesquiterpene jet fuel blendstocks Geiselman, Gina M.; Kirby, James; Landera, Alexander figshare https://doi.org/10.6084/m9.figshare.c.5206392	collection	January 2020
Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass Kirby, James; Geiselman, Gina M.; Yaegashi, Junko figshare https://doi.org/10.6084/m9.figshare.c.5397705	collection	January 2021

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