Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach

Deaver, Noland R.; Hesse, Cedar; Kuske, Cheryl Rae; Porras-Alfaro, Andrea

doi:10.1016/j.funbio.2019.09.006

Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach

Journal Article · Mon Sep 23 00:00:00 EDT 2019 · Fungal Biology

DOI:https://doi.org/10.1016/j.funbio.2019.09.006· OSTI ID:1569616

Deaver, Noland R. ^[1]; Hesse, Cedar ^[2]; ^[3]; ^[1]

Western Illinois Univ., Macomb, IL (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States); United States Department of Agriculture, Corvallis, OR (United States)
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

For decades entomopathogenic fungi have garnered interest as possible alternatives to chemical pesticides. However, their ecology outside of agroecosystems demands further study. We assessed the diversity and abundance of entomopathogenic and insect-associated fungi at a loblolly pine forest in North Carolina, USA using culture-dependent and next-generation sequencing libraries. Fungi were isolated using Galleria mellonella larvae, as well as from soil dilutions plated on a selective medium. Isolates were identified using Sanger sequencing of the ITS and LSU rRNA gene regions, and represented 36 OTUs including Metarhizium, Lecanicillium, and Paecilomyces. Additionally, we assessed the chitinolytic potential of isolates and found widespread, variable ability to degrade chitin within and between genera. Phylogenetic analyses resolved several isolates to genus, with some forming clades with other insect-associated taxa, as well as with fungi associated with plant tissues. Saprophytes were widely distributed in soil, while entomopathogens were less abundant and present primarily in the top two cm of the soil. Furthermore, the similarity between culture-dependent and next-generation sequencing results demonstrates that both methods can be used concurrently in this system to study the ecology of entomopathogenic fungi.

View Accepted Manuscript (DOE)

Research Organization:: Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: 89233218CNA000001

OSTI ID:: 1569616

Alternate ID(s):: OSTI ID: 1780389

Report Number(s):: LA-UR--19-20465

Journal Information:: Fungal Biology, Journal Name: Fungal Biology Journal Issue: 12 Vol. 123; ISSN 1878-6146

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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