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Title: DHAD variants and methods of screening

Patent ·
OSTI ID:1568323
;

Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

Research Organization:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AR0000006
Assignee:
Butamax Advanced Biofuels LLC (Wilmington, DE)
Patent Number(s):
10,287,566
Application Number:
15/406,876
OSTI ID:
1568323
Resource Relation:
Patent File Date: 01/16/2017
Country of Publication:
United States
Language:
English

References (99)

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Ketol-acid reductoisomerase using NADH patent March 2012
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