A size-invariant bud-duration timer enables robustness in yeast cell size control
- Marine Biological Lab., Woods Hole, MA (United States); Geisel School of Medicine at Dartmouth, Hanover, NH (United States)
- Marine Biological Lab., Woods Hole, MA (United States); Max Planck Inst. for the Physics of Complex Systems, Max Planck Inst. of Molecular Cell Biology and Genetics, Dresden (Germany). Center for Systems Biology Dresden
- Marine Biological Lab., Woods Hole, MA (United States); Univ. of California, San Francisco, CA (United States)
- Marine Biological Lab., Woods Hole, MA (United States); Princeton Univ., NJ (United States)
- Univ. of Lausanne, Lausanne (Switzerland); editor
Cell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant 'timer' specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.
- Research Organization:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- Grant/Contract Number:
- AC02-05CH11231
- OSTI ID:
- 1561878
- Journal Information:
- PLoS ONE, Vol. 13, Issue 12; ISSN 1932-6203
- Publisher:
- Public Library of ScienceCopyright Statement
- Country of Publication:
- United States
- Language:
- English
Web of Science
Similar Records
Relationship between sensitivity to ultraviolet light and budding in yeast cells of different culture ages
The TCP4 transcription factor of Arabidopsis blocks cell division in yeast at G1 {yields} S transition