Relationship between sensitivity to ultraviolet light and budding in yeast cells of different culture ages

Journal Article · Mon Mar 01 00:00:00 EST 1976 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574387· OSTI ID:7364222

Atsuta, J; Okajima, S

Subpopulations of yeast cells, consisting of cells of different sizes and different percentages of budding cells, were prepared by centrifugation through sucrose solutions with linear density gradients of cultures at different phases of the growth cycle. Ultraviolet survival of these cells was determined by colony counting, and the survival rate was compared with the cells' respiratory rates. Individual budding cells and interdivisional cells, and also mother cells and daughter cells derived from irradiated budding cells, were isolated by the micromanipulation technique. The number of divisions in each cell was measured during a 21-hr incubation period immediately after irradiation. In the population in the logarithmic phase consisting of homogeneous cells of middle size, no difference in uv sensitivity was observed between mother cells and daughter cells, irrespective of mutual adhesion. Budding cell resistance was observed in the population in the transitional phase; this was due to the lesser uv sensitivity of daughter cells in the fresh medium. In the stationary phase, daughter cells were rather more sensitive than mother cells or interdivisional cells, so there was little difference in uv sensitivity between budding cells and interdivisional cells. (auth)

• Research Organization: Nagasaki Univ.
• OSTI ID: 7364222
• Journal Information: Radiat. Res.; (United States), Vol. 65:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CELL DIVISION
RADIOSENSITIVITY EFFECTS
SACCHAROMYCES CEREVISIAE
RADIOSENSITIVITY
BIOLOGICAL RADIATION EFFECTS
IRRADIATION
SURVIVAL TIME
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
BIOMASS
ELECTROMAGNETIC RADIATION
ENERGY SOURCES
FUNGI
MICROORGANISMS
PLANTS
RADIATION EFFECTS
RADIATIONS
RENEWABLE ENERGY SOURCES
SACCHAROMYCES
YEASTS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)