Insulator Nanostructure Desorption Ionization Mass Spectrometry

Journal Article · Thu Jul 19 04:00:00 EDT 2018 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.8b01989· OSTI ID:1545139

Duncombe, Todd A. ^[1]; Raad, Markus De ^[2]; Bowen, Benjamin P. ^[3]; Singh, Anup K. ^[1]; Northen, Trent R. ^[4]

---

1. DOE Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, California 94608, United States; Sandia National Laboratories, Livermore, California 94550, United States
2. Environmental Genomics and Systems Biology, Biosciences, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, United States
3. Joint Genome Institute, Department of Energy, 2800 Mitchell Drive, Walnut Creek, California 94598, United States; Environmental Genomics and Systems Biology, Biosciences, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, United States
4. DOE Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, California 94608, United States; Joint Genome Institute, Department of Energy, 2800 Mitchell Drive, Walnut Creek, California 94598, United States; Environmental Genomics and Systems Biology, Biosciences, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, United States

Surface-assisted laser desorption ionization (SALDI) is an approach for gas-phase ion generation for mass spectrometry using laser excitation on typically conductive or semiconductive nanostructures. Here, we introduce insulator nanostructure desorption ionization mass spectrometry (INDI-MS), a nanostructured polymer substrate for SALDI-MS analysis of small molecules and peptides. INDI-MS surfaces are produced through the self-assembly of a perfluoroalkyl silsesquioxane nanostructures in a single chemical vapor deposition silanization-step. We find that surfaces formed from the perfluorooctyltrichlorosilane monomer assemble semielliptical features with a 10 nm height, diameters between 10 and 50 nm, and have attomole-femtomole sensitivities for selected analytes. Surfaces prepared with silanes that either lack the trichloro or perfluoro groups, lack sensitivity. Further, we demonstrate that hydrophobic INDI regions can be micropatterned onto hydrophilic surfaces to perform on-chip self-desalting in an array format.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 1545139
• Journal Information: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 16 Vol. 90; ISSN 0003-2700
• Country of Publication: United States
• Language: English