Metagenomic insights into diazotrophic communities across Arctic glacier forefields

Nash, Maisie V.; Anesio, Alexandre M.; Barker, Gary; Tranter, Martyn; Varliero, Gilda; Eloe-Fadrosh, Emiley A.; Nielsen, Torben; Turpin-Jelfs, Thomas; Benning, Liane G.; Sánchez-Baracaldo, Patricia

doi:10.1093/femsec/fiy114

Metagenomic insights into diazotrophic communities across Arctic glacier forefields

Journal Article · Tue Jun 12 00:00:00 EDT 2018 · FEMS Microbiology Ecology (Online)

DOI:https://doi.org/10.1093/femsec/fiy114· OSTI ID:1461009

Nash, Maisie V. ^[1]; Anesio, Alexandre M. ^[1]; Barker, Gary ^[2]; Tranter, Martyn ^[1]; Varliero, Gilda ^[2]; Eloe-Fadrosh, Emiley A. ^[3]; Nielsen, Torben ^[3]; Turpin-Jelfs, Thomas ^[1]; Benning, Liane G. ^[4]; Sánchez-Baracaldo, Patricia ^[1]

School of Geographical Sciences, University of Bristol, UK
School of Life Sciences, University of Bristol, UK
DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, US
GFZ German Research Centre for Geosciences, Telegrafenenberg, 14473 Potsdam, Germany, School of Earth and Environment, University of Leeds, LS2 9JT, Leeds, UK, Department of Earth Sciences, Free University of Berlin, Malteserstr, 74-100, Building A, 12249, Berlin, Germany

Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes.

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Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1461009

Alternate ID(s):: OSTI ID: 1543956

Journal Information:: FEMS Microbiology Ecology (Online), Journal Name: FEMS Microbiology Ecology (Online) Journal Issue: 9 Vol. 94; ISSN 1574-6941

Publisher:: Federation of European Microbiological SocietiesCopyright Statement

Country of Publication:: Netherlands

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Arctic
Microbiology
diazotrophs
diversity
forefield
metagenomics
nitrogen fixation

Metagenomic insights into diazotrophic communities across Arctic glacier forefields

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