Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy

Prigozhin, Maxim B.; Maurer, Peter C.; Courtis, Alexandra M.; Liu, Nian; Wisser, Michael D.; Siefe, Chris; Tian, Bining; Chan, Emory; Song, Guosheng; Fischer, Stefan; Aloni, Shaul; Ogletree, D. Frank; Barnard, Edward S.; Joubert, Lydia-Marie; Rao, Jianghong; Alivisatos, A. Paul; Macfarlane, Roger M.; Cohen, Bruce E.; Cui, Yi; Dionne, Jennifer A.; Chu, Steven

doi:10.1038/s41565-019-0395-0

Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy

Journal Article · Sun Mar 03 23:00:00 EST 2019 · Nature Nanotechnology

DOI:https://doi.org/10.1038/s41565-019-0395-0· OSTI ID:1542401

Prigozhin, Maxim B. ^[1]; Maurer, Peter C. ^[1]; ^[2]; ^[3]; Wisser, Michael D. ^[1]; Siefe, Chris ^[1]; Tian, Bining ^[4]; Chan, Emory ^[4]; Song, Guosheng ^[5]; Fischer, Stefan ^[1]; Aloni, Shaul ^[4]; Ogletree, D. Frank ^[4]; ^[4]; ^[6]; Rao, Jianghong ^[1]; Alivisatos, A. Paul ^[7]; Macfarlane, Roger M. ^[8]; ^[4]; ^[1]; Dionne, Jennifer A. ^[1] more »

Stanford Univ., CA (United States)
Univ. of California, Berkeley, CA (United States)
Stanford Univ., CA (United States); Georgia Inst. of Technology, Atlanta, GA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Stanford Univ., CA (United States); Hunan Univ., Changsha (China)
Stanford Univ., CA (United States); Stellenbosch Univ. (South Africa)
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
IBM Research, San Jose, CA (United States)

Electron microscopy has been integral in our understanding of complex biological systems. Although electron microscopy reveals cellular morphology with nanoscale resolution, it does not provide information on the location of different types of proteins. An electron-microscopy-based bioimaging technology capable of localizing individual proteins and resolving protein-protein interactions with respect to cellular ultrastructure would provide important insights into the molecular biology of a cell. In this report, we synthesize small lanthanide-doped nanoparticles and measure the absolute photon emission rate of individual nanoparticles resulting from a given electron excitation flux (cathodoluminescence). Our results indicate that the optimization of nanoparticle composition, synthesis protocols and electron imaging conditions can lead to sub-20-nm nanolabels that would enable high signal-to-noise localization of individual biomolecules within a cellular context. In ensemble measurements, these labels exhibit narrow spectra of nine distinct colours, so the imaging of biomolecules in a multicolour electron microscopy modality may be possible.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Light-Material Interactions in Energy Conversion (LMI); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH), National Science Foundation (NSF)

Grant/Contract Number:: AC02-05CH11231; SC0001293

OSTI ID:: 1542401

Journal Information:: Nature Nanotechnology, Journal Name: Nature Nanotechnology Journal Issue: 5 Vol. 14; ISSN 1748-3387

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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