Single Particle Cathodoluminescence Spectroscopy with Sub-20 nm, Electron-Stable Phosphors

Swearer, Dayne F.; Fischer, Stefan; Angell, Daniel K.; Siefe, Chris; Alivisatos, A. Paul; Chu, Steven; Dionne, Jennifer A.

doi:10.1021/acsphotonics.1c00235

Single Particle Cathodoluminescence Spectroscopy with Sub-20 nm, Electron-Stable Phosphors

Journal Article · Mon May 17 00:00:00 EDT 2021 · ACS Photonics

DOI:https://doi.org/10.1021/acsphotonics.1c00235· OSTI ID:1840947

^[1]; ^[1]; ^[1]; Siefe, Chris ^[1]; ^[2]; Chu, Steven ^[3]; ^[4]

Stanford Univ. School of Engineering, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States); Kavli Energy Nanoscience Inst., Berkeley, CA (United States)
Stanford Univ., CA (United States)
Stanford Univ. School of Engineering, CA (United States); Stanford Univ. School of Medicine, CA (United States)

Lanthanide-doped nanophosphors have emerged as promising optical labels for high-resolution, "multicolor"electron microscopy. Here, we develop a library of 11 unique lanthanide-doped nanophosphors with average edge lengths of 15.2 ± 2.0 nm (N = 4284). These nanophosphors consist of an electron-stable BaYF5 host lattice doped at 25% atomic concentration with the lanthanides Pr³⁺, Nd³⁺, Sm³⁺, Eu³⁺, Tb³⁺, Dy³⁺, Ce³⁺, Ho³⁺, Er³⁺, Tm³⁺, and Yb³⁺. Under ~100 pA/nm² beam beam current in a transmission electron microscope, each nanophosphor species exhibits strong cathodoluminescence spectra with sharp characteristic emission lines for each lanthanide. The bright emission and stability of these nanoparticles enable not only ensemble, but also single-particle cathodoluminescence spectroscopy, which we demonstrate with BaYF₅:Ln³⁺, where Ln³⁺ = Tb³⁺, Ho³⁺, Er³⁺, Sm³⁺, Eu³⁺, or Pr³⁺. Single-particle cathodoluminescence corresponds directly with HAADF intensity across nanoparticles, confirming high spatial localization of the measured cathodoluminescence signal of lanthanide-doped nanophosphors. Furthermore, our synthesis and characterization of sub-20 nm, electron-stable nanophosphors provides a robust material platform to achieve single-molecule labeled correlative cathodoluminescence electron microscopy, a critical foundation for high-resolution correlation of single molecules within the context of cellular ultrastructure.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1840947

Journal Information:: ACS Photonics, Journal Name: ACS Photonics Journal Issue: 6 Vol. 8; ISSN 2330-4022

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
cathodoluminescence
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Single Particle Cathodoluminescence Spectroscopy with Sub-20 nm, Electron-Stable Phosphors

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