A Selection for Assembly Reveals That a Single Amino Acid Mutant of the Bacteriophage MS2 Coat Protein Forms a Smaller Virus-like Particle

Asensio, Michael A.; Morella, Norma M.; Jakobson, Christopher M.; Hartman, Emily C.; Glasgow, Jeff E.; Sankaran, Banumathi; Zwart, Peter H.; Tullman-Ercek, Danielle

doi:10.1021/acs.nanolett.6b02948

A Selection for Assembly Reveals That a Single Amino Acid Mutant of the Bacteriophage MS2 Coat Protein Forms a Smaller Virus-like Particle

Journal Article · Tue Aug 23 00:00:00 EDT 2016 · Nano Letters

DOI:https://doi.org/10.1021/acs.nanolett.6b02948· OSTI ID:1532201

Asensio, Michael A. ^[1]; Morella, Norma M. ^[1]; Jakobson, Christopher M. ^[1]; Hartman, Emily C. ^[1]; Glasgow, Jeff E. ^[1]; Sankaran, Banumathi ^[2]; Zwart, Peter H. ^[2]; Tullman-Ercek, Danielle ^[3]

Univ. of California, Berkeley, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Northwestern University, Evanston, IL (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Virus-like particles are used to encapsulate drugs, imaging agents, enzymes, and other biologically active molecules in order to enhance their function. However, the size of most virus-like particles is inflexible, precluding the design of appropriately sized containers for different applications. Here, we describe a chromatographic selection for virus-like particle assembly. Using this selection, we identified a single amino acid substitution to the coat protein of bacteriophage MS2 that mediates a uniform switch in particle geometry from T = 3 to T = 1 icosahedral symmetry. The resulting smaller particle retains the ability to be disassembled and reassembled in vitro and to be chemically modified to load cargo into its interior cavity. Here, the pair of 27 and 17 nm MS2 particles will allow direct examination of the effect of size on function in established applications of virus-like particles, including drug delivery and imaging.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1532201

Journal Information:: Nano Letters, Journal Name: Nano Letters Journal Issue: 9 Vol. 16; ISSN 1530-6984

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Molecular determinants of large cargo transport into the nucleus Paci, Giulia; Lemke, Edward A. bioRxiv https://doi.org/10.1101/695080	posted_content	July 2019
Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids Martín Garrido, Natàlia; Crone, Michael A.; Ramlaul, Kailash Molecular Microbiology, Vol. 113, Issue 1 https://doi.org/10.1111/mmi.14406	journal	November 2019

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