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Affinity selection of Nipah and Hendra virus-related vaccine candidates from a complex random peptide library displayed on bacteriophage virus-like particles

Patent ·
OSTI ID:1340588
; ; ; ;
The invention relates to virus-like particles of bacteriophage MS2 (MS2 VLPs) displaying peptide epitopes or peptide mimics of epitopes of Nipah Virus envelope glycoprotein that elicit an immune response against Nipah Virus upon vaccination of humans or animals. Affinity selection on Nipah Virus-neutralizing monoclonal antibodies using random sequence peptide libraries on MS2 VLPs selected peptides with sequence similarity to peptide sequences found within the envelope glycoprotein of Nipah itself, thus identifying the epitopes the antibodies recognize. The selected peptide sequences themselves are not necessarily identical in all respects to a sequence within Nipah Virus glycoprotein, and therefore may be referred to as epitope mimics VLPs displaying these epitope mimics can serve as vaccine. On the other hand, display of the corresponding wild-type sequence derived from Nipah Virus and corresponding to the epitope mapped by affinity selection, may also be used as a vaccine.
Research Organization:
Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC04-94AL85000
Assignee:
STC.UNM (Albuquerque, NM)
Patent Number(s):
9,549,976
Application Number:
14/081,629
OSTI ID:
1340588
Country of Publication:
United States
Language:
English

References (6)

Immunogenic display of diverse peptides, including a broadly cross-type neutralizing human papillomavirus L2 epitope, on virus-like particles of the RNA bacteriophage PP7 journal June 2010
BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences journal May 1999
Roles of operator and non-operator RNA sequences in bacteriophage R17 capsid assembly journal December 1988
Subunit Fusion Confers Tolerance to Peptide Insertions in a Virus Coat Protein journal November 1997
Peptide Epitope Identification by Affinity Selection on Bacteriophage MS2 Virus-Like Particles journal June 2011
Immunogenic Display of Diverse Peptides on Virus-like Particles of RNA Phage MS2 journal June 2008

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES