Biochip reader with enhanced illumination and bioarray positioning apparatus

Title: Biochip reader with enhanced illumination and bioarray positioning apparatus

Abstract

A method of illumination and illumination apparatus are provided in a biochip reader. Illumination is provided by a non-collimated laser source or a light emitting diode (LED). The light is directed to opposing sides of a glass substrate by a pair of optical fiber bundles. The glass substrate carries a bioarray. Each of the optical fiber bundles are splayed out to make a fan, the fan being one fiber thick and defining a line of optical fiber faces. This process randomizes any non-uniformity in the illumination source, creating a more uniform illumination source. A respective divergent diffuser engages each row of optical fiber faces coupling and diffusing light substantially evenly through the opposing sides of the glass substrate to illuminate the bioarray supported by the glass substrate. The glass substrate functions as a secondary light guide. The divergent diffusers separate the optical fiber faces from the edges of the glass substrate, protecting the optical fibers from mechanical damage. A glass holder supports the glass substrate carrying the bioarray. The glass holder including a plastics springs member in spring contact engagement with the glass substrate for positioning said bioarray in a focal plane. Light also can be directed to opposing endsmore »« less

Inventors:: Yershov, Gennadiy; Alferov, Oleg; Kukhtin, Alexander

Publication Date:: 2003-09-16

Research Org.:: Univ. of Chicago, IL (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1531462

Patent Number(s):: 6,620,623

Application Number:: 10/139,842

Assignee:: The University of Chicago (Chicago, IL)

DOE Contract Number:: W-31-109-ENG-38

Resource Type:: Patent

Resource Relation:: Patent File Date: 2002-05-06

Country of Publication:: United States

Language:: English

Subject:: 42 ENGINEERING; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS

Citation Formats


                    Yershov, Gennadiy, Alferov, Oleg, and Kukhtin, Alexander. Biochip reader with enhanced illumination and bioarray positioning apparatus.  United States: N. p., 2003. 
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                    Yershov, Gennadiy, Alferov, Oleg, & Kukhtin, Alexander. Biochip reader with enhanced illumination and bioarray positioning apparatus.  United States.

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                    Yershov, Gennadiy, Alferov, Oleg, and Kukhtin, Alexander. Tue .  
        "Biochip reader with enhanced illumination and bioarray positioning apparatus".  United States.  https://www.osti.gov/servlets/purl/1531462.

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@article{osti_1531462,

  title        = {Biochip reader with enhanced illumination and bioarray positioning apparatus},

  author       = {Yershov, Gennadiy and Alferov, Oleg and Kukhtin, Alexander},

  abstractNote = {A method of illumination and illumination apparatus are provided in a biochip reader. Illumination is provided by a non-collimated laser source or a light emitting diode (LED). The light is directed to opposing sides of a glass substrate by a pair of optical fiber bundles. The glass substrate carries a bioarray. Each of the optical fiber bundles are splayed out to make a fan, the fan being one fiber thick and defining a line of optical fiber faces. This process randomizes any non-uniformity in the illumination source, creating a more uniform illumination source. A respective divergent diffuser engages each row of optical fiber faces coupling and diffusing light substantially evenly through the opposing sides of the glass substrate to illuminate the bioarray supported by the glass substrate. The glass substrate functions as a secondary light guide. The divergent diffusers separate the optical fiber faces from the edges of the glass substrate, protecting the optical fibers from mechanical damage. A glass holder supports the glass substrate carrying the bioarray. The glass holder including a plastics springs member in spring contact engagement with the glass substrate for positioning said bioarray in a focal plane. Light also can be directed to opposing ends of the glass substrate by a second pair of optical fiber bundles. Also a single optical fiber bundle can be used to direct light in one side of the glass substrate or three optical fiber bundles can be used to direct light into the glass substrate. This method of illumination provides a superior signal to noise ratio as compared with conventional illumination systems.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2003},

  month        = {9}

}

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Works referenced in this record:

Devices for molecular biological analysis and diagnostics including waveguides
patent, November 2001

Ackley, Donald E.; Butler, William F.; Swanson, Paul
US Patent Document 6,315,953
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6315953

Biochip detection system
patent, August 2001

Watson, Jr., Robert Malcolm; Chaudhry, Haseeb R.; Lee, Jr., James S.
US Patent Document 6,271,042
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6271042

Manufacturing DNA microarrays of high spot homogeneity and reduced background signal
journal, April 2001

Diehl, F.
Nucleic Acids Research, Vol. 29, Issue 7
DOI: 10.1093/nar/29.7.e38

Methods for concurrently processing multiple biological chip assays
patent, February 1999

Rava, Richard P.; Fodor, Stephen; Trulson, Mark O.
US Patent Document 5,874,219
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5874219

Arrangement for optically reading out the information from substrates having a multiplicity of individual samples
patent, April 2003

Thorwirth, Guenter; Reiland, Werner
US Patent Document 6,542,241
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6542241

Method and device for fluorescence measurement
patent, May 2003

Yurino, Noriko; Yamamoto, Kenji; Shishido, Kentaro
US Patent Document 6,563,584
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6563584

Optical scanning apparatus
patent, December 1996

Dorsel, Andreas N.; Sampas, Nicholas M.
US Patent Document 5,585,639
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5585639

Darkfield illuminator for a microscope slide
patent, September 1993

Laronga, Victor; Thorburn, Stanley
US Patent Document 5,249,077
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=5249077

Works referencing / citing this record:

Optical detecting apparatus for a bio-chip
patent, August 2012

Cho, Seongho
US Patent Document 8,246,912
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8246912

Substrate holders for uniform reactive sputtering
patent, May 2016

Nolan, Thomas
US Patent Document 9,343,273
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=9343273

Modular, micro-scale, optical array and biodetection system
patent, December 2008

Yershov, Gennadiy M.
US Patent Document 7,463,353
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7463353

Scanner with array anti-degradation features
patent, July 2007

Corson, John F.; Parker, Russell A.
US Patent Document 7,247,494
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7247494

Optical scanning configurations, systems, and methods involving at least one actuator for scanning a scan head
patent, October 2009

Hoshizaki, Jon A.; King, Howard G.; Sluis, Johannes P.
US Patent Document 7,599,060
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7599060

Apparatus for and method of measuring bio-chips using uniform total internal reflection illumination
patent, September 2011

Kang, Uk; Bae, Soo Jin; Papayan, Geri V.
US Patent Document 8,017,079
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8017079

Molecular reader
patent, May 2013

Knopp, Kevin J.; Vakhshoori, Daryoosh; Wang, Peidong
US Patent Document 8,449,842
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8449842

Apparatus for and method of measuring bio-chips using uniform total internal reflection illumination
patent, June 2012

Kang, Uk; Bae, Soo Jin; Papayan, Geri V.
US Patent Document 8,192,686
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=8192686

Optical instrument including excitation source
patent, March 2016

Boege, Steven J.; Oldham, Mark F.; Young, Eugene F.
US Patent Document 9,285,318
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=9285318

Scanning system and method for scanning a plurality of samples
patent, November 2005

Oldham, Mark F.; Young, Eugene F.
US Patent Document 6,965,105
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6965105

Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
patent, September 2008

Hoshizaki, Jon A.; King, Howard Gregg; Sluis, Johannes P.
US Patent Document 7,423,750
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=7423750

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Title: Biochip reader with enhanced illumination and bioarray positioning apparatus

Abstract

Citation Formats

Devices for molecular biological analysis and diagnostics including waveguides patent, November 2001

Biochip detection system patent, August 2001

Manufacturing DNA microarrays of high spot homogeneity and reduced background signal journal, April 2001

Methods for concurrently processing multiple biological chip assays patent, February 1999

Arrangement for optically reading out the information from substrates having a multiplicity of individual samples patent, April 2003

Method and device for fluorescence measurement patent, May 2003

Optical scanning apparatus patent, December 1996

Darkfield illuminator for a microscope slide patent, September 1993

Optical detecting apparatus for a bio-chip patent, August 2012

Substrate holders for uniform reactive sputtering patent, May 2016

Modular, micro-scale, optical array and biodetection system patent, December 2008

Scanner with array anti-degradation features patent, July 2007

Optical scanning configurations, systems, and methods involving at least one actuator for scanning a scan head patent, October 2009

Apparatus for and method of measuring bio-chips using uniform total internal reflection illumination patent, September 2011

Molecular reader patent, May 2013

Apparatus for and method of measuring bio-chips using uniform total internal reflection illumination patent, June 2012

Optical instrument including excitation source patent, March 2016

Scanning system and method for scanning a plurality of samples patent, November 2005

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Devices for molecular biological analysis and diagnostics including waveguides
patent, November 2001

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patent, August 2001

Manufacturing DNA microarrays of high spot homogeneity and reduced background signal
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Methods for concurrently processing multiple biological chip assays
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Arrangement for optically reading out the information from substrates having a multiplicity of individual samples
patent, April 2003

Method and device for fluorescence measurement
patent, May 2003

Optical scanning apparatus
patent, December 1996

Darkfield illuminator for a microscope slide
patent, September 1993

Optical detecting apparatus for a bio-chip
patent, August 2012

Substrate holders for uniform reactive sputtering
patent, May 2016

Modular, micro-scale, optical array and biodetection system
patent, December 2008

Scanner with array anti-degradation features
patent, July 2007

Optical scanning configurations, systems, and methods involving at least one actuator for scanning a scan head
patent, October 2009

Apparatus for and method of measuring bio-chips using uniform total internal reflection illumination
patent, September 2011

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patent, May 2013

Apparatus for and method of measuring bio-chips using uniform total internal reflection illumination
patent, June 2012

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patent, March 2016

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patent, November 2005

Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
patent, September 2008