Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating

Ziemba, Christopher; Khavkin, Maria; Priftis, Dimitris; Acar, Handan; Mao, Jun; Benami, Maya; Gottlieb, Moshe; Tirrell, Matthew; Kaufman, Yair; Herzberg, Moshe

doi:10.1021/acs.langmuir.8b00181

Title: Antifouling Properties of a Self-Assembling Glutamic Acid-Lysine Zwitterionic Polymer Surface Coating

Journal Article · Wed Apr 11 00:00:00 EDT 2018 · Langmuir

DOI:https://doi.org/10.1021/acs.langmuir.8b00181· OSTI ID:1514874

Ziemba, Christopher ^[1]; Khavkin, Maria ^[1]; Priftis, Dimitris ^[2]; Acar, Handan ^[2];

^[2]; Benami, Maya ^[1]; Gottlieb, Moshe ^[1]; Tirrell, Matthew ^[3]; Kaufman, Yair ^[1];

^[1]

Ben-Gurion University of the Negev (BGU), Beersheba (Israel)
Univ. of Chicago, IL (United States)
Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Lemont, IL (United States)

There is a necessity for the development of antifouling materials to resist adsorption of biomacromolecules. Here we describe the preparation of a novel zwitterionic block copolymer with the potential to prevent or delay the formation of microbial biofilms. The block copolymer comprised a zwitterionic (hydrophilic) section of alternating glutamic acid (negatively charged) and lysine (positively charged) units and a hydrophobic polystyrene section. Cryo-TEM and dynamic light-scattering (DLS) results showed that, on average, the block copolymer self-assembled into 7-nm-diameter micelles in aqueous solutions (0 to 100 mM NaCl, pH 6). Quartz crystal microbalance with dissipation monitoring (QCM-D), atomic force microscopy (AFM), and contact angle measurements demonstrated that the block copolymer self-assembled into a brush-like monolayer on polystyrene surfaces. The brush-like monolayer produced from a 100 mg/L block copolymer solution exhibited an average distance, d, of approximately 4-8 nm between each block copolymer molecule (center to center). Once the brush-like monolayer self-assembled, it reduced EPS adsorption onto the polystyrene surface by similar to 70% (mass), reduced the rate of bacterial attachment by >80%, and inhibited the development of thick biofilms. QCM-D results revealed that the EPS molecules penetrate between the chains of the brush and adsorb onto the polystyrene surface. Additionally, AFM analyses showed that the brush-like monolayer prevents the adhesion of large (>d) hydrophilic colloids onto the surface via hydration repulsion; yet, molecules or colloids small enough to fit between the brush polymers (

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: Ben-Gurion University of the Negev (BGU), Beersheba (Israel)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1514874

Journal Information:: Langmuir, Vol. 35, Issue 5; ISSN 0743-7463

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 14 works

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