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Genomic Comparison of Two Family-Level Groups of the Uncultivated NAG1 Archaeal Lineage from Chemically and Geographically Disparate Hot Springs

Journal Article · · Frontiers in Microbiology
 [1];  [2];  [3];  [4];  [5];  [6];  [4];  [5]
  1. Northern Illinois Univ., DeKalb, IL (United States); Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States); Department of Biological Sciences, Northern Illinois University, DeKalb, IL (United States)
  2. California State Univ. (CalState), San Bernardino, CA (United States); Univ. of Nevada, Las Vegas, NV (United States)
  3. Univ. of Nevada, Las Vegas, NV (United States); Université Paris-Saclay, Jouy-en-Josas (France). Institut National de la Recherche Agronomique (INRA)
  4. Univ. of Nevada, Las Vegas, NV (United States)
  5. Northern Illinois Univ., DeKalb, IL (United States)
  6. Bigelow Lab. for Ocean Sciences, East Boothbay, ME (United States)
+ Show Author Affiliations
Recent progress based on single-cell genomics and metagenomic investigations of archaea in a variety of extreme environments has led to significant advances in our understanding of the diversity, evolution, and metabolic potential of archaea, yet the vast majority of archaeal diversity remains undersampled. In this work, we coordinated single-cell genomics with metagenomics in order to construct a near-complete genome from a deeply branching uncultivated archaeal lineage sampled from Great Boiling Spring (GBS) in the U.S. Great Basin, Nevada. This taxon is distantly related (distinct families) to an archaeal genome, designated “Novel Archaeal Group 1” (NAG1), which was extracted from a metagenome recovered from an acidic iron spring in Yellowstone National Park (YNP). We compared the metabolic predictions of the NAG1 lineage to better understand how these archaea could inhabit such chemically distinct environments. Similar to the NAG1 population previously studied in YNP, the NAG1 population from GBS is predicted to utilize proteins as a primary carbon source, ferment simple carbon sources, and use oxygen as a terminal electron acceptor under oxic conditions. However, GBS NAG1 populations contained distinct genes involved in central carbon metabolism and electron transfer, including nitrite reductase, which could confer the ability to reduce nitrite under anaerobic conditions. Despite inhabiting chemically distinct environments with large variations in pH, GBS NAG1 populations shared many core genomic and metabolic features with the archaeon identified from YNP, yet were able to carve out a distinct niche at GBS.
Research Organization:
Nevada System of Higher Education (NSHE), Las Vegas, NV (United States); Univ. of California, Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231; EE0000716
OSTI ID:
1510510
Journal Information:
Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 8; ISSN 1664-302X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (2)

Position-Specific Metabolic Probing and Metagenomics of Microbial Communities Reveal Conserved Central Carbon Metabolic Network Activities at High Temperatures journal July 2019
Asgard archaea are diverse, ubiquitous, and transcriptionally active microbes posted_content July 2018

Figures / Tables (3)

Table 1(p. 4)table Table 1
Figure 1(p. 5)figure Figure 1
Figure 2(p. 6)figure Figure 2

Similar Records

Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park
Journal Article · Thu Feb 28 23:00:00 EST 2013 · The ISME Journal, 7(3):622-634 · OSTI ID:1064570

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Great Boiling Spring
NAG1 lineage
extreme microbiology
microbial ecology
uncultivated archaea