Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly

Wang, Shen; Li, Yun; Gong, Jihong; Ye, Sheng; Yang, Xiaofei; Zhang, Rongguang; Ma, Cong

doi:10.1038/s41467-018-08028-6

Title: Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly

Journal Article · Tue Jan 08 00:00:00 EST 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-08028-6· OSTI ID:1510246

^[1]; Li, Yun ^[1]; Gong, Jihong ^[1]; Ye, Sheng ^[2]; Yang, Xiaofei ^[3]; Zhang, Rongguang ^[4]; Ma, Cong ^[1]

Huazhong Univ. of Science and Technology, Wuhan (China)
Chinese Academy of Sciences (CAS), Beijing (China)
South-Central Univ. for Nationalities, Wuhan (China)
Chinese Academy of Sciences (CAS), Beijing (China); Chinese Academy of Sciences, Shanghai (China)

The transition of the Munc18-1/syntaxin-1 complex to the SNARE complex, a key step involved in exocytosis, is regulated by Munc13-1, SNAP-25 and synaptobrevin-2, but the underlying mechanism remains elusive. Here, we identify an interaction between Munc13-1 and the membrane-proximal linker region of synaptobrevin-2, and reveal its essential role in transition and exocytosis. Upon this interaction, Munc13-1 not only recruits synaptobrevin-2-embedded vesicles to the target membrane but also renders the synaptobrevin-2 SNARE motif more accessible to the Munc18-1/syntaxin-1 complex. Afterward, the entry of SNAP-25 leads to a half-zippered SNARE assembly, which eventually dissociates the Munc18-1/syntaxin-1 complex to complete SNARE complex formation. Furthermore, our data suggest that Munc18-1 and Munc13-1 together serve as a functional template to orchestrate SNARE complex assembly.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Natural Science Foundation of China (NSFC); National Key Basic Research Program of China

Grant/Contract Number:: 31670846; 31721002; 31670850; 2015CB910800

OSTI ID:: 1510246

Journal Information:: Nature Communications, Vol. 10, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 252 works

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