Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming

Yang, Xiaoyu; Wang, Shen; Sheng, Yi; Zhang, Mingshu; Zou, Wenjuan; Wu, Lijie; Kang, Lijun; Rizo, Josep; Zhang, Rongguang; Xu, Tao; Ma, Cong

doi:10.1038/nsmb.3038

Syntaxin opening by the MUN domain underlies the function of Munc13 in synaptic-vesicle priming

Journal Article · Mon Jun 01 00:00:00 EDT 2015 · Nature Structural & Molecular Biology

DOI:https://doi.org/10.1038/nsmb.3038· OSTI ID:1259896

Yang, Xiaoyu ^[1]; ^[1]; Sheng, Yi ^[1]; Zhang, Mingshu ^[2]; Zou, Wenjuan ^[3]; Wu, Lijie ^[4]; Kang, Lijun ^[3]; Rizo, Josep ^[5]; Zhang, Rongguang ^[6]; Xu, Tao ^[7]; Ma, Cong ^[1]

Huazhong Univ. of Science and Technology, Wuhan (China)
Chinese Academy of Sciences (CAS), Beijing (China)
Zhejiang Univ., Hangzhou (China)
Chinese Academy of Sciences (CAS), Shanghai (China)
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
Chinese Academy of Sciences (CAS), Beijing (China); Chinese Academy of Sciences (CAS), Shanghai (China)
Huazhong Univ. of Science and Technology, Wuhan (China); Chinese Academy of Sciences (CAS), Beijing (China); Univ. of Chinese Academy of Sciences, Beijing (China)

UNC-13-Munc13s have a central function in synaptic-vesicle priming through their MUN domains. However, it is unclear whether this function arises from the ability of the MUN domain to mediate the transition from the Munc18-1–closed syntaxin-1 complex to the SNARE complex in vitro. The crystal structure of the rat Munc13-1 MUN domain now reveals an elongated, arch-shaped architecture formed by α-helical bundles, with a highly conserved hydrophobic pocket in the middle. Mutation of two residues (NF) in this pocket abolishes the stimulation caused by the Munc13-1 MUN domain on SNARE-complex assembly and on SNARE-dependent proteoliposome fusion in vitro. Moreover, the same mutation in UNC-13 abrogates synaptic-vesicle priming in Caenorhabditis elegans neuromuscular junctions. Here, these results support the notion that orchestration of syntaxin-1 opening and SNARE-complex assembly underlies the central role of UNC-13-Munc13s in synaptic-vesicle priming.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Key Basic Research Program of China; National Science Foundation of China; U.S. National Inst. of Health

OSTI ID:: 1259896

Journal Information:: Nature Structural & Molecular Biology, Journal Name: Nature Structural & Molecular Biology Journal Issue: 7 Vol. 22; ISSN 1545-9993

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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