Capillary Isoelectric Focusing–Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry for Protein Characterization

Journal Article · Sat Aug 01 04:00:00 EDT 1998 · Analytical Chemistry

DOI:https://doi.org/10.1021/ac980224o· OSTI ID:1508279

Yang, Liyu; Lee, Cheng S.; Hofstadler, Steven A.; Pasa-Tolic, Ljiljana; Smith, Richard D.

In this study, on-line combination of capillary isoelectric focusing (CIEF) with ESI-FTICR is presented for the characterization of model proteins, human hemoglobin variants, and Escherichia coli proteins. The focusing effect of CIEF further permits analysis of dilute protein samples, with a typical 50-100 fold concentrating factor. With the full genome of several microorganisms having been sequenced and the sequencing of human genome well underway, capabilities for the analysis of the corresponding proteomes are attracting increased attention. Two-dimensional analysis of cellular proteins using CIEF-ESI-FTICR provides a major step toward the comprehensive characterization of complex biological processes such as development, differentiation, and signal transduction in the field of cellular biochemistry.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1508279
• Report Number(s): PNNL-SA-29818
• Journal Information: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 15 Vol. 70; ISSN 0003-2700
• Publisher: American Chemical Society (ACS)
• Country of Publication: United States
• Language: English

Related Subjects

electrospray ionization
FTICR