On-line capillary isoelectric focusing-electrospray mass spectrometry for protein characterization
- Ames Lab., IA (United States)
The integration of capillary isoelectric focusing (CIEF) with electrospray mass spectrometry (ESMS) as a two-dimensional separation system for protein characterization will be presented. Mixtures of protein variants are focused and cathodically mobilized in a polyacrylamide coated capillary. At the end of CIEF capillary, the mobilized protein zones are analyzed by mass spectrometry coupled on-line to an electrospray interface with a coaxial sheath flow configuration. The effects of carrier ampholyte concentration on the CIEF separation and the protein electrospray mass spectra will be discussed. On-line CIEF-ESMS with superior resolving power, speed, and sensitivity will be demonstrated for the analysis of hemoglobin and glycoprotein variants.
- OSTI ID:
- 539073
- Report Number(s):
- CONF-960807--
- Country of Publication:
- United States
- Language:
- English
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