Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

MBP-binding DARPins facilitate the crystallization of an MBP fusion protein

Journal Article · · Acta Crystallographica. Section F, Structural Biology Communications
; ;

The production of high-quality crystals is the main bottleneck in determining the structures of proteins using X-ray crystallography. In addition to being recognized as a very effective solubility-enhancing fusion partner,Escherichia colimaltose-binding protein (MBP) has also been successfully employed as a `fixed-arm' crystallization chaperone in more than 100 cases. Here, it is reported that designed ankyrin-repeat proteins (DARPins) that bind with high affinity to MBP can promote the crystallization of an MBP fusion protein when the fusion protein alone fails to produce diffraction-quality crystals. As a proof of principle, three different co-crystal structures of MBP fused to the catalytic domain of human dual-specificity phosphatase 1 in complex with DARPins are reported.

Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
National Institutes of Health (NIH)
OSTI ID:
1506492
Journal Information:
Acta Crystallographica. Section F, Structural Biology Communications, Vol. 74, Issue 9; ISSN 2053-230X
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
ENGLISH

References (26)

Protein-protein crystal-packing contacts October 1997
A Dominant Conformational Role for Amino Acid Diversity in Minimalist Protein–Protein Interfaces August 2008
A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1 April 2015
The family-wide structure and function of human dual-specificity protein phosphatases January 2014
Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation: Sgf29 functions as an H3K4me2/3 binder in SAGA June 2011
HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes July 2006
Enhanced Crystallizability by Protein Engineering Approaches: A General Overview May 2012
MolProbity : all-atom structure validation for macromolecular crystallography December 2009
Coot model-building tools for molecular graphics November 2004
Towards automated crystallographic structure refinement with phenix.refine March 2012
PROCHECK: a program to check the stereochemical quality of protein structures April 1993
The structure of the dust mite allergen Der p 7 reveals similarities to innate immune proteins April 2010
Crystal structures of MBP fusion proteins: Structures of MBP Fusion Proteins January 2016
Toward a Molecular Understanding of the Interaction of Dual Specificity Phosphatases with Substrates: Insights from Structure-Based Modeling and High Throughput Screening October 2008
Protein crystallization by rational mutagenesis of surface residues: Lys to Ala mutations promote crystallization of RhoGDI April 2001
Crystal structure of the human dual specificity phosphatase 1 catalytic domain: Crystal Structure of the DUSP1 Catalytic Domain November 2017
Protein crystallization by surface entropy reduction: optimization of the SER strategy April 2007
Inference of Macromolecular Assemblies from Crystalline State September 2007
LigPlot+: Multiple Ligand–Protein Interaction Diagrams for Drug Discovery October 2011
High-affinity binders selected from designed ankyrin repeat protein libraries April 2004
The protein as a variable in protein crystallization April 2003
Crystal structure of the catalytic domain of human MKP-2 reveals a 24-mer assembly August 2009
Design of an expression system to enhance MBP-mediated crystallization January 2017
Phaser crystallographic software July 2007
To fuse or not to fuse: What is your purpose?: Protein Fusion Technology September 2013
Distinct Binding Determinants for ERK2/p38α and JNK MAP Kinases Mediate Catalytic Activation and Substrate Selectivity of MAP Kinase Phosphatase-1 January 2001

Similar Records

MBP-binding DARPins facilitate the crystallization of an MBP fusion protein
Journal Article · 2018 · Acta Crystallographica. Section F, Structural Biology Communications · OSTI ID:1467532
Structure, function, and evolution of bacterial ATP-binding cassette systems
Journal Article · 2010 · Microbiol. Mol. Biol R. · OSTI ID:1007155
+1 more
Galectin-1 as a fusion partner for the production of soluble and folded human {beta}-1,4-galactosyltransferase-T7 in E. coli
Journal Article · 2010 · Biochemical and Biophysical Research Communications · OSTI ID:22202464

Related Subjects