A kinetic study on the bioremediation of sodium cyanide and acetonitrile by free and immobilized cells of pseudomonas putida
Conference
·
OSTI ID:150455
- Selma Univ., AL (United States); and others
Pseudomonas putida capable of utilizing organic nitrile (acetonitrile) and inorganic cyanide (sodium cyanide) as the sole source of carbon and nitrogen was isolated from contaminated industrial sites and waste water. The bacterium possesses nitrile aminohydrolase (EC 3.5.5.1) and amidase (EC 3.5.1.4), which are involved in the transformation of cyanides and nitrites into ammonia and CO{sub 2} through the formation of amide as an intermediate. Both of the enzymes have a high selectivity and affinity toward the {sup -}CN group. The rate of degradation of acetonitrile and sodium cyanide to ammonia and CO{sub 2} by the calcium-alginate immobilized cells of P. putida was studied. The rate of reaction during the biodegradation of acetonitrile and sodium cyanide, and the substrate- and product-dependent kinetics of these toxic compounds were studied using free and immobilized cells of P. putida and modeled using a simple Michaelis-Menten equation.
- Research Organization:
- Oak Ridge National Lab., TN (United States)
- OSTI ID:
- 150455
- Report Number(s):
- CONF-940526--
- Country of Publication:
- United States
- Language:
- English
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