Lactic acid production by pellet-form Rhizopus oryzae in a submerged system
- Purdue Univ., West Lafayette, IN (United States)
Rhizopus oryzae NRRL 395 produces optically pure L(+)-lactic acid that is highly preferred for the production of environmentally benign polymers. With xylose as the carbon source for cultivation, it can be self-immobilized as pellets with a size of about 1 mm. Repeated usage of the same pellets by transferring them into fresh media every time when the glucose was exhausted over a period of 22 d yielded 1742 or 2001 g/L lactic acid total (based on 100-mL working volume) depending on the media used. Lactic acid is known to be a strong inhibitor for both growth and production, and it can be removed continuously by the adsorption on the PVP resin. With the fermenter-adsorber system, the fermentation can be performed as effectively as the ones with added neutralizing agents, such as calcium carbonate and sodium hydroxide. One problem of the fermenter-adsorber system is that lower production was obtained than in shake flasks; hence, proper reactor design is necessary to improve the process.
- Research Organization:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- OSTI ID:
- 150395
- Report Number(s):
- CONF-940526-; TRN: 95:007973-0007
- Resource Relation:
- Conference: 16. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 9-13 May 1994; Other Information: PBD: 1995; Related Information: Is Part Of Sixteenth symposium on biotechnology for fuels and chemicals; Davison, B.H. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [National Renewable Energy Lab., Golden, CO (United States)]; PB: 823 p.
- Country of Publication:
- United States
- Language:
- English
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