Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

Gao, Johnway; Skeen, Rodney S

Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

Patent · 03 March 2003

OSTI ID:875089

Gao, Johnway ^[1]; Skeen, Rodney S ^[2]

Richland, WA
Pendleton, OR

The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 2 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

DOE Contract Number:: AC06-76RL01830

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Number(s):: 6,528,636

OSTI ID:: 875089

Country of Publication:: United States

Language:: English

References (12)

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Enhanced production of l(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor Yin, Peimin; Nishina, Naoki; Kosakai, Yuuko Journal of Fermentation and Bioengineering, Vol. 84, Issue 3 https://doi.org/10.1016/S0922-338X(97)82063-6	journal	January 1997
Gene Transfer by Electroporation of Filamentous Fungi Kapoor, M. Electroporation Protocols for Microorganisms, p. 279-289 https://doi.org/10.1385/0-89603-310-4:279	book	June 1995
Sequence of the Penicillium chrysogenum phosphoglycerate kinase gene Solingen, Pieter van; Muurling, Hilde; Koekman, Bert Nucleic Acids Research, Vol. 16, Issue 24 https://doi.org/10.1093/nar/16.24.11823	journal	January 1988
Isolation and identification of yeast messenger ribonucleic acids coding for enolase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate kinase Holland, Michael J.; Holland, Janice P. Biochemistry, Vol. 17, Issue 23, p. 4900-4907 https://doi.org/10.1021/bi00616a007	journal	November 1978
The primary structure of the Saccharomyces cerevisiae gene for 3-phosphogrycerate kinase Hitzeman, Ronald A.; Hagie, Frank E.; Hayflick, Joei S. Nucleic Acids Research, Vol. 10, Issue 23 https://doi.org/10.1093/nar/10.23.7791	journal	January 1982
Production of l-lactic acid by Rhizopus species Soccol, C. R.; Stonoga, V. I.; Raimbault, M. World Journal of Microbiology & Biotechnology, Vol. 10, Issue 4 https://doi.org/10.1007/BF00144467	journal	July 1994
Transformation of Filamentous Fungi with Plasmid DNA by Electroporation Richey, M. G. Phytopathology, Vol. 79, Issue 8 https://doi.org/10.1094/Phyto-79-844	journal	January 1989
Promoter structure and expression of the 3-phosphoglycerate kinase-encoding gene (pgk1) of Trichoderma reesei Vanhanen, S.; Saloheimo, A.; Ilmén, M. Gene, Vol. 106, Issue 1 https://doi.org/10.1016/0378-1119(91)90577-X	journal	September 1991
Analysis of the 3-phosphoglycerate kinase 2 promoter in Rhizopus niveus Takaya, Naoki; Yanai, Koji; Horiuchi, Hiroyuki Gene, Vol. 152, Issue 1 https://doi.org/10.1016/0378-1119(94)00679-M	journal	January 1995
Cloning and characterization of two 3-phosphoglycerate kinase genes of Rhizopus niveus and heterologous gene expression using their promoters Takaya, Naoki; Yanai, Koji; Horiuchi, Hiroyuki Current Genetics, Vol. 25, Issue 6 https://doi.org/10.1007/BF00351673	journal	June 1994
Cloning, expression and characterization of a cDNA encoding a lipase from Rhizopus delemar Haas, Michael J.; Allen, John; Berka, Thomas R. Gene, Vol. 109, Issue 1 https://doi.org/10.1016/0378-1119(91)90594-2	journal	December 1991

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/999/435/
3-phosphoglycerate
acid-producing
addition
carbohydrate
clone
conditions
constitutively
design
discovery
expressing
expression
filamentous
foreign
fungal
fungus
integration
isolated
kinase
lactic
lactic acid
method
oryzae
phosphoglycerate
promoter
promoter sequence
provides
regulate
rhizopus
sequence
species
strain
transformation
vector

Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

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