3-D Multispectral Monitoring of Living-Cell Signaling Using Confocal-Imaging and FPGA Processing

Conference · Mon May 24 04:00:00 EDT 2004

OSTI ID:15020446

Resat, Marianne S; Solinsky, James C; Wiley, H S; Perrine, Kenneth A; Seim, Thomas A; Budge, Scott E

Online control of living cell signal propagation experiments is being facilitated with field programmable gate array (FPGA) processing. The technology approach captures confocal fluorescence microscopy imagery in a manner that real-time, live-cell chemical signaling can be monitored during an experiment for extended time periods. The experiments obtain quantitative, spatial characteristics of the cell chemistry from the imagery through analysis, following a localized perturbation stimulus. The construction of a high-speed, confocal microscope provides simultaneous, two color image acquisition, at speeds of up to 30 fps, allowing near-real-time fluorescent resonance energy transfer and subsequent ratiometric, chemical sigaling analysis. FPGA provides real-time image processing hardware for obsering adjustments of the microscope settings as the images are received, using real-time image registration and fusion algorithms displayed through a user interface.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 15020446
• Report Number(s): PNNL-SA-41577; 3680
• Country of Publication: United States
• Language: English

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