Modeling and simulation of DNA flow in a microfluidic-based pathogen detection system

Conference · Mon Jan 31 04:00:00 EST 2005

DOI:https://doi.org/10.1109/MMB.2005.1548471· OSTI ID:15016241

Trebotich, D; Miller, G H

We present simulation results from a new computational model of DNA flow in microfluidic devices. This work is important because computational models are needed to design miniaturized biomedical devices that are becoming the state-of-the-art in many significant applications including pathogen detection as well as continuous monitoring and drug delivery. Currently advanced algorithms in design tools are non-existent but necessary to understand the complex fluid and polymer dynamics involved in biological flow at small scales. Our model is based on a fully coupled fluid-particle numerical algorithm with both stochastic and deterministic components in a bead-rod polymer representation. We have applied this work to DNA extraction configurations in a microfluidic PCR chamber used in a pathogen detection system. We demonstrate our method on the test problem of flow of a single DNA molecule in a 2D packed array microchannel. We are also investigating mechanisms for molecular ''sticking'' using short range forces.

• Research Organization: Lawrence Livermore National Lab., Livermore, CA (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 15016241
• Report Number(s): UCRL-CONF-209334
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
74 ATOMIC AND MOLECULAR PHYSICS
99 GENERAL AND MISCELLANEOUS
ALGORITHMS
DESIGN
DETECTION
DNA
MONITORING
PATHOGENS
POLYMERS
SIMULATION