Energetics and Dynamics of Peptide Fragmentation from Multiple-Collision Activation and Surface-Induced Dissociation Studies

Journal Article · Mon Mar 29 04:00:00 EST 2004 · European Journal of Mass Spectrometry, 10(2):259-267

DOI:https://doi.org/10.1255/ejms.641· OSTI ID:15007487

Laskin, Julia

This account summarizes the energetics and dynamics of peptide fragmentation obtained using a new approach recently developed in our laboratory. The approach involves RRKM modeling of time- and energy-resolved MS/MS data obtained using collisional activation. We demonstrate that surface-induced dissociation (SID) on a long timescale of Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) is perfectly suited for studying the energetics and dynamics of peptide fragmentation. The advantages provided by SID include very fast ion activation, which eliminates possible discrimination against higher-energy dissociation pathways, and efficient amplification of small changes in dissociation parameters. We present a summary of results obtained for small alanine-containing peptides as well as larger peptides including angiotensin analogs and a series of peptides containing the LDIFSDF motif.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 15007487
• Report Number(s): PNNL-SA-39098; 6291; KC0302020
• Journal Information: European Journal of Mass Spectrometry, 10(2):259-267, Journal Name: European Journal of Mass Spectrometry, 10(2):259-267
• Country of Publication: United States
• Language: English

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