Identification of Sporulated and Vegetative Bacteria using Statistical Analysis of Fourier Transform Mid-Infrared Transmission Data
A combined mid-infrared spectroscopic/statistical modeling approach for the discrimination and identification, at the strain level, of both sporulated and vegetative bacterial samples is presented. Transmission mode spectra of bacteria dried on ZnSe windows were collected using a Fourier-transform mid-infrared (FTIR) spectrometer. Five Bacillus bacterial strains (B. atrophaeus 49337, B. globigii, B. thuringiensis ssp. kurstaki, B. subtilis 49780, and B. subtilis 6051) were used to construct a reference spectral library and to parameterize a four-step statistical model for the systematic identification of bacteria. The statistical methods used included principal-component analysis (PCA), classification and regression trees (CART), and Mahalanobis-distance calculations. Internal cross-validation studies successfully classified 100% of the samples into their correct physiological state (sporulated or vegetative) and identified 67% of the samples correctly as to their bacterial strain. Analysis of thirteen blind samples, which included reference and other bacteria, nonbiological materials, and mixtures of both nonbiological and bacterial samples, yielded comparable accuracy. The chief advantage of this approach is the accurate identification of unknown bacteria, including spores, in a matter of minutes.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 15007110
- Report Number(s):
- PNNL-SA-38461; APSPA4; 5116; TRN: US200414%%222
- Journal Information:
- Applied Spectroscopy, 58(2):203-211, Vol. 58, Issue 2; ISSN 0003-7028
- Country of Publication:
- United States
- Language:
- English
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