FTIR Transmission and Photoacoustic Spectroscopy for the Statistical Identification of Bacteria

Conference · Fri Mar 12 00:00:00 EST 2004

OSTI ID:15007637

Foster, Nancy S; Valentine, Nancy B; Thompson, Sandra E; Johnson, Timothy J; Amonette, James E

We have previously reported a combined mid-infrared spectroscopic/statistical modeling approach for the discrimination and identification, at the strain level, of both sporulated and vegetative bacteria. This paper reports on the expansion of the reference spectral library: transmissive Fourier-transform mid-infrared (trans-FTIR) spectra were obtained for three Escherichia bacterial strains (E. coli RZ1032, E. coli W3110, and E. coli HB101 ATCC 33694), and two Pseudomonas putida bacterial strains (P. putida 0301 and P. putida ATCC 39169). These were combined with the previous spectral data of five Bacillus bacterial strains (B. atrophaeus ATCC 49337, B. globigii Dugway, B. thuringiensis spp. kurstaki ATCC 35866, B. subtilis ATCC 49760, and B. subtilis 6051) to form an extended library. The previously developed four step statistical model for the identification of bacteria (using the expanded library) was subsequently used on blind samples including other bacteria as well as non-biological materials. The results from the trans-FTIR spectroscopy experiments are discussed and compared to results obtained using photoacoustic Fourier-transform infrared spectroscopy (PA-FTIR). The advantages, disadvantages, and preliminary detection limits for each technique are discussed. Both methods yield promising identification of unknown bacteria, including bacterial spores, in a matter of minutes.

• Research Organization: Pacific Northwest National Lab., Richland, WA (US), Environmental Molecular Sciences Laboratory (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 15007637
• Report Number(s): PNNL-SA-39817; 5116; TRN: US200419%%395
• Resource Relation: Conference: Proceedings of SPIE - Chemical and Biological Point Sensors for Homeland Defense, Conference location not supplied, Conference dates not supplied; Other Information: PBD: 12 Mar 2004
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACILLUS
BACTERIA
BACTERIAL SPORES
PHOTOACOUSTIC SPECTROSCOPY
PSEUDOMONAS
SENSITIVITY
SIMULATION
SPECTRA
SPECTROSCOPY
STATISTICAL MODELS
STRAINS
ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY
FTIR
PHOTOACOUSTIC
SPECTROSCOPY
CHEMOMETRICS
BACTERIA
SPORES
BACILLUS
ESCHERICHIA
PSEUDOMONAS