Combined Confocal and Magnetic Resonance Microscopy

Journal Article · Sun May 12 04:00:00 EDT 2002 · Applied Magnetic Resonance, 22(2):145-158

DOI:https://doi.org/10.1007/BF03166099· OSTI ID:15001400

Wind, Robert A; Majors, Paul D; Minard, Kevin R; Ackerman, Eric J; Daly, Don S; Holtom, Gary R; Thrall, Brian D; Weber, Thomas J

Confocal and magnetic resonance microscopy are both used to study live cells in a minimally invasive way. Both techniques provide complementary information. Therefore, by examining cells simultaneously with both methodologies, more detailed information is obtained than is possible with each of the microscopes individually. In this paper two configurations of a combined confocal and magnetic resonance microscope described. In both cases the sample compartment is part of a temperature regulated perfusion system. The first configuration is capable of studying large single cells or three-dimensional cell agglomerates, whereas with the second configuration monolayers of mammalian cells can be investigated . Combined images are shown of Xenopus laevis frog oocytes, model JB6 tumor spheroids, and a single layer of Chinese hamster ovary cells. Finally, potential applications of the combined microscope are discussed.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 15001400
• Report Number(s): PNNL-SA-35466; KP1402010
• Journal Information: Applied Magnetic Resonance, 22(2):145-158, Journal Name: Applied Magnetic Resonance, 22(2):145-158 Journal Issue: 2 Vol. 22
• Country of Publication: United States
• Language: English

Related Subjects

47 OTHER INSTRUMENTATION
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CHO CELLS
DESIGN
FROGS
MAGNETIC RESONANCE
MICROSCOPES
MICROSCOPY
NEOPLASMS
OOCYTES
SPHEROIDS