Design and Performance of an ESI Interface for Selective External Ion Accumulation Coupled to a Fourier Transform Ion Cyclotron Mass Spectrometer

Journal Article · Fri Dec 01 00:00:00 EST 2000 · Analytical Chemistry

OSTI ID:15001373

Belov, Mikhail E ^[1]; Nikolaev, Eugene N ^[2]; Anderson, Gordon A ^[1]; Udseth, Harold R ^[1]; Conrads, Thomas P ^[1]; Veenstra, Timothy D ^[1]; Masselon, Christophe D ^[1]; Gorshkov, Michael V ^[2]; Smith, Richard D ^[1]

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1. BATTELLE (PACIFIC NW LAB)
2. ASSOC WESTERN UNIVERSITY

The coupling of Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) with electrospray ionization (ESI) has advanced the analysis of large byopolymers. In this work the combination of need for high throughput protein characterization (e.g. for rapid and proteome analyses), high-performance capillary liquid chromatography (HPLC) with FTICR mass spectrometry and external ion accumulation has been shown to increase in both sensitivity and analysis duty cycle. Instrument versatility is further improved by ion pre-selection followed by ion accumulation in an external linear quadrupole ion trap. The interface was tested with a 3.5 tesla FTICR mass spectrometer and evaluated with a number of peptides and proteins whose molecular weights ranged from 500 Da to 66 kDa. A significant increase in the sensitivity, duty cycle and dynamic range over that of the previously used accumulated trapping was achieved, exhibiting a detection limit of {approx}10 zmol ({approx}6000 molecules) for smaller proteins such as cytochrome c. Capillary LC external accumulation interface with FTICR was successfully applied for the study of the tryptic digest of mouse proteome.

• Research Organization: Pacific Northwest National Lab., Richland, WA (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 15001373
• Report Number(s): PNNL-SA-33327; KP1301030; TRN: US0400301
• Journal Information: Analytical Chemistry, Vol. 73, Issue 2; Other Information: PBD: 1 Dec 2000
• Country of Publication: United States
• Language: English

Related Subjects

43 PARTICLE ACCELERATORS
CHROMATOGRAPHY
CYCLOTRONS
CYTOCHROMES
ION CYCLOTRON-RESONANCE
IONIZATION
MASS SPECTROMETERS
MASS SPECTROSCOPY
MOLECULAR WEIGHT
PEPTIDES
PERFORMANCE
PROTEINS
QUADRUPOLES
SENSITIVITY
TRAPPING