Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration

Park, Kyoungwon; Kuo, Yung; Shvadchak, Volodymyr; Ingargiola, Antonino; Dai, Xinghong; Hsiung, Lawrence; Kim, Wookyeom; Zhou, Hong; Zou, Peng; Levine, Alex J.; Li, Jack; Weiss, Shimon

doi:10.1126/sciadv.1601453

Title: Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration

Journal Article · Fri Jan 12 00:00:00 EST 2018 · Science Advances

DOI:https://doi.org/10.1126/sciadv.1601453· OSTI ID:1499965

Park, Kyoungwon ^[1];

^[1];

^[2];

^[1]; Dai, Xinghong ^[3];

^[4]; Kim, Wookyeom ^[5];

^[6]; Zou, Peng ^[7]; Levine, Alex J. ^[8]; Li, Jack ^[1];

^[9]

Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry
Academy of Sciences of the Czech Republic (ASCR), Prague (Czech Republic). Inst. of Organic Chemistry and Biochemistry
Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics
Univ. of California, Los Angeles, CA (United States). Dept. of Chemical and Biomolecular Engineering
Univ. of California, Los Angeles, CA (United States). Dept. of Ecology and Evolutionary Biology
Univ. of California, Los Angeles, CA (United States). Dept. of Microbiology, Immunology, and Molecular Genetics. California NanoSystems Inst.
Harvard Univ., Cambridge, MA (United States). Dept. of Chemistry and Chemical Biology
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. Dept. of Physics
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry. California NanoSystems Inst. Dept. of Physiology

We developed membrane voltage nanosensors that are based on inorganic semiconductor nanoparticles. We provide here a feasibility study for their utilization. We use a rationally designed peptide to functionalize the nanosensors, imparting them with the ability to self-insert into a lipid membrane with a desired orientation. Once inserted, these nanosensors could sense membrane potential via the quantum confined Stark effect, with a single-particle sensitivity. With further improvements, these nanosensors could potentially be used for simultaneous recording of action potentials from multiple neurons in a large field of view over a long duration and for recording electrical signals on the nanoscale, such as across one synapse.

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Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Inst. of Health (NIH) (United States); National Science Foundation (NSF); Defense Advanced Research Projects Agency (DARPA) (United States); United States–Israel Binational Science Foundation; Human Frontier Science Program

Grant/Contract Number:: FC02-02ER63421; AC02-05CH11231; 1S10RR23057; GM071940; DMR-1309188; DMR-1548924; D14PC00141; 2010382; RGP0061/2015

OSTI ID:: 1499965

Journal Information:: Science Advances, Vol. 4, Issue 1; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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