Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry
Georg-August-Univ., Gottingen (Germany). III. Inst. of Physics-Biophysics
Weizmann Inst. of Science, Rehovot (Israel)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Foundry
Univ. of California, Los Angeles, CA (United States). Dept. of Chemistry and Biochemistry, California NanoSystems Inst., and Dept. of Physiology; Bar-Ilan Univ., Ramat-Gan (Israel). Dept. of Physics, Inst. for Nanotechnology and Advanced Materials
We optimized the performance of quantum-confined Stark effect (QCSE)-based voltage nanosensors. A high-throughput approach for single-particle QCSE characterization was developed and utilized to screen a library of such nanosensors. Type-II ZnSe/CdS-seeded nanorods were found to have the best performance among the different nanosensors evaluated in this work. The degree of correlation between intensity changes and spectral changes of the exciton's emission under an applied field was characterized. An upper limit for the temporal response of individual ZnSe/CdS nanorods to voltage modulation was characterized by high-throughput, high temporal resolution intensity measurements using a novel photon-counting camera. The measured 3.5 μs response time is limited by the voltage modulation electronics and represents ~30 times higher bandwidth than needed for recording an action potential in a neuron.
Kuo, Yung, et al. "Characterizing the Quantum-Confined Stark Effect in Semiconductor Quantum Dots and Nanorods for Single-Molecule Electrophysiology." ACS Photonics, vol. 5, no. 12, Oct. 2018. https://doi.org/10.1021/acsphotonics.8b00617
Kuo, Yung, Li, Jack, Michalet, Xavier, et al., "Characterizing the Quantum-Confined Stark Effect in Semiconductor Quantum Dots and Nanorods for Single-Molecule Electrophysiology," ACS Photonics 5, no. 12 (2018), https://doi.org/10.1021/acsphotonics.8b00617
@article{osti_1650039,
author = {Kuo, Yung and Li, Jack and Michalet, Xavier and Chizhik, Alexey and Meir, Noga and Bar-Elli, Omri and Chan, Emory and Oron, Dan and Enderlein, Joerg and Weiss, Shimon},
title = {Characterizing the Quantum-Confined Stark Effect in Semiconductor Quantum Dots and Nanorods for Single-Molecule Electrophysiology},
annote = {We optimized the performance of quantum-confined Stark effect (QCSE)-based voltage nanosensors. A high-throughput approach for single-particle QCSE characterization was developed and utilized to screen a library of such nanosensors. Type-II ZnSe/CdS-seeded nanorods were found to have the best performance among the different nanosensors evaluated in this work. The degree of correlation between intensity changes and spectral changes of the exciton's emission under an applied field was characterized. An upper limit for the temporal response of individual ZnSe/CdS nanorods to voltage modulation was characterized by high-throughput, high temporal resolution intensity measurements using a novel photon-counting camera. The measured 3.5 μs response time is limited by the voltage modulation electronics and represents ~30 times higher bandwidth than needed for recording an action potential in a neuron.},
doi = {10.1021/acsphotonics.8b00617},
url = {https://www.osti.gov/biblio/1650039},
journal = {ACS Photonics},
issn = {ISSN 2330-4022},
number = {12},
volume = {5},
place = {United States},
publisher = {American Chemical Society (ACS)},
year = {2018},
month = {10}}
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES); Defense Advanced Research Projects Agency (DARPA); European Research Council (ERC); Human Frontier Science Program (HFSP); National Science Foundation (NSF)
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 567, Issue 1https://doi.org/10.1016/j.nima.2006.05.155