An Isoprene Lipid-Binding Protein Promotes Eukaryotic Coenzyme Q Biosynthesis

Lohman, Danielle C.; Aydin, Deniz; Von Bank, Helaina C.; Smith, Robert W.; Linke, Vanessa; Weisenhorn, Erin; McDevitt, Molly T.; Hutchins, Paul; Wilkerson, Emily M.; Wancewicz, Benjamin; Russell, Jason; Stefely, Matthew S.; Beebe, Emily T.; Jochem, Adam; Coon, Joshua J.; Bingman, Craig A.; Dal Peraro, Matteo; Pagliarini, David J.

doi:10.1016/j.molcel.2018.11.033

An Isoprene Lipid-Binding Protein Promotes Eukaryotic Coenzyme Q Biosynthesis

Journal Article · Wed Jan 16 23:00:00 EST 2019 · Molecular Cell

DOI:https://doi.org/10.1016/j.molcel.2018.11.033· OSTI ID:1497168

Lohman, Danielle C. ^[1]; Aydin, Deniz ^[2]; Von Bank, Helaina C. ^[3]; Smith, Robert W. ^[4]; Linke, Vanessa ^[4]; Weisenhorn, Erin ^[4]; McDevitt, Molly T. ^[4]; Hutchins, Paul ^[5]; Wilkerson, Emily M. ^[4]; Wancewicz, Benjamin ^[4]; Russell, Jason ^[6]; Stefely, Matthew S. ^[1]; Beebe, Emily T. ^[4]; Jochem, Adam ^[3]; Coon, Joshua J. ^[7]; Bingman, Craig A. ^[1]; Dal Peraro, Matteo ^[2]; Pagliarini, David J. ^[1]

Morgridge Inst. for Research, Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
Ecole Polytechnique Federale Lausanne (EPFL), Lausanne (Switzlerland)
Morgridge Inst. for Research, Madison, WI (United States)
Univ. of Wisconsin, Madison, WI (United States)
Univ. of Wisconsin, Madison, WI (United States); Genome Center of Wisconsin, Madison, WI (United States)
Morgridge Inst. for Research, Madison, WI (United States); Genome Center of Wisconsin, Madison, WI (United States)
Morgridge Inst. for Research, Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States); Genome Center of Wisconsin, Madison, WI (United States)

The biosynthesis of coenzyme Q presents a paradigm for how cells surmount hydrophobic barriers in lipid biology. In eukaryotes, CoQ precursors—among nature’s most hydrophobic molecules—must somehow be presented to a series of enzymes peripherally associated with the mitochondrial inner membrane. Here in this paper, we reveal that this process relies on custom lipid-binding properties of COQ9. We show that COQ9 repurposes the bacterial TetR fold to bind aromatic isoprenes with high specificity, including CoQ intermediates that likely reside entirely within the bilayer. We reveal a process by which COQ9 associates with cardiolipin-rich membranes and warps the membrane surface to access this cargo. Finally, we identify a molecular interface between COQ9 and the hydroxylase COQ7, motivating a model whereby COQ9 presents intermediates directly to CoQ enzymes. Overall, our results provide a mechanism for how a lipid-binding protein might access, select, and deliver specific cargo from a membrane to promote biosynthesis.

View Accepted Manuscript (DOE)

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF); Swiss National Science Foundation (SNSF)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1497168

Journal Information:: Molecular Cell, Journal Name: Molecular Cell Journal Issue: 4 Vol. 73; ISSN 1097-2765

Publisher:: Cell Press - ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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