Direct Writing of Tunable Living Inks for Bioprocess Intensification

Qian, Fang; Zhu, Cheng; Knipe, Jennifer M.; Ruelas, Samantha; Stolaroff, Joshuah K.; DeOtte, Joshua R.; Duoss, Eric B.; Spadaccini, Christopher M.; Henard, Calvin A.; Guarnieri, Michael T.; Baker, Sarah E.

doi:10.1021/acs.nanolett.9b00066

Title: Direct Writing of Tunable Living Inks for Bioprocess Intensification

Journal Article · Thu Jan 31 00:00:00 EST 2019 · Nano Letters

DOI:https://doi.org/10.1021/acs.nanolett.9b00066· OSTI ID:1495251

; Zhu, Cheng;

; Ruelas, Samantha; Stolaroff, Joshuah K.; DeOtte, Joshua R. ^[1]; Duoss, Eric B.; Spadaccini, Christopher M.; Henard, Calvin A. ^[2]; Guarnieri, Michael T. ^[2]; Baker, Sarah E.

University of California, Davis, California 95616, United States
National Bioenergy Center, National Renewable Energy Laboratory, Golden, Colorado 80401, United States

Critical to the success of three-dimensional (3D) printing of living materials with high performance is the development of new ink materials and 3D geometries that favor long-term cell functionality. Here we report the use of freeze-dried live cells as the solid filler to enable a new living material system for direct ink writing of catalytically active microorganisms with tunable densities and various self-supporting porous 3D geometries. Baker’s yeast was used as an exemplary live whole-cell biocatalyst, and the printed structures displayed high resolution, large scale, high catalytic activity and long-term viability. An unprecedented high cell loading was achieved, and cell inks showed unique thixotropic behavior. In the presence of glucose, printed bioscaffolds exhibited increased ethanol production compared to bulk counterparts due largely to improved mass transfer through engineered porous structures. The new living materials developed in this work could serve as a versatile platform for process intensification of an array of bioconversion processes utilizing diverse microbial biocatalysts for production of high-value products or bioremediation applications.

View Journal Article

Cite

Export

Save

Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC36-08GO28308; AC52-07NA27344

OSTI ID:: 1495251

Alternate ID(s):: OSTI ID: 1500077; OSTI ID: 1508842; OSTI ID: 1592032

Report Number(s):: NREL/JA-5100-73418; LLNL-JRNL-753559

Journal Information:: Nano Letters, Journal Name: Nano Letters Vol. 19 Journal Issue: 9; ISSN 1530-6984

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 52 works

Citation information provided by
Web of Science

References (25)

A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice Laronda, Monica M.; Rutz, Alexandra L.; Xiao, Shuo Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15261	journal	May 2017
3D Bioprinting of Vascularized, Heterogeneous Cell-Laden Tissue Constructs Kolesky, David B.; Truby, Ryan L.; Gladman, A. Sydney Advanced Materials, Vol. 26, Issue 19 https://doi.org/10.1002/adma.201305506	journal	February 2014
Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer Denby, Charles M.; Li, Rachel A.; Vu, Van T. Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-03293-x	journal	March 2018
3D printing of bacteria into functional complex materials Schaffner, Manuel; Rühs, Patrick A.; Coulter, Fergal Science Advances, Vol. 3, Issue 12 https://doi.org/10.1126/sciadv.aao6804	journal	December 2017
Instability-Assisted Direct Writing of Microstructured Fibers Featuring Sacrificial Bonds Passieux, Renaud; Guthrie, Leigh; Rad, Somayeh Hosseini Advanced Materials, Vol. 27, Issue 24 https://doi.org/10.1002/adma.201500603	journal	May 2015
Complete biosynthesis of noscapine and halogenated alkaloids in yeast Li, Yanran; Li, Sijin; Thodey, Kate Proceedings of the National Academy of Sciences, Vol. 115, Issue 17 https://doi.org/10.1073/pnas.1721469115	journal	April 2018
Engineered 3D-printed artificial axons Espinosa-Hoyos, Daniela; Jagielska, Anna; Homan, Kimberly A. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-017-18744-6	journal	January 2018
Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches Liu, Xiaonan; Cheng, Jian; Zhang, Guanghui Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-02883-z	journal	January 2018
3D Bioprinting Human Induced Pluripotent Stem Cell Constructs for In Situ Cell Proliferation and Successive Multilineage Differentiation Gu, Qi; Tomaskovic-Crook, Eva; Wallace, Gordon G. Advanced Healthcare Materials, Vol. 6, Issue 17 https://doi.org/10.1002/adhm.201700175	journal	May 2017
3D bioprinting of tissues and organs Murphy, Sean V.; Atala, Anthony Nature Biotechnology, Vol. 32, Issue 8 https://doi.org/10.1038/nbt.2958	journal	August 2014
Direct-Write Assembly of 3D Hydrogel Scaffolds for Guided Cell Growth Barry, Robert A.; Shepherd, Robert F.; Hanson, Jennifer N. Advanced Materials, Vol. 21, Issue 23 https://doi.org/10.1002/adma.200803702	journal	June 2009
Production of freeze‐dried yeast culture for the brewing of traditional sorghum beer, tchapalo N'Guessan, Florent K.; Coulibaly, Hermann W.; Alloue‐Boraud, Mireille W. A. Food Science & Nutrition, Vol. 4, Issue 1 https://doi.org/10.1002/fsn3.256	journal	July 2015
Bioprinting the Cancer Microenvironment Zhang, Yu Shrike; Duchamp, Margaux; Oklu, Rahmi ACS Biomaterials Science & Engineering, Vol. 2, Issue 10 https://doi.org/10.1021/acsbiomaterials.6b00246	journal	May 2016
3D Microperiodic Hydrogel Scaffolds for Robust Neuronal Cultures Hanson Shepherd, Jennifer N.; Parker, Sara T.; Shepherd, Robert F. Advanced Functional Materials, Vol. 21, Issue 1 https://doi.org/10.1002/adfm.201001746	journal	November 2010
Cytocompatibility studies of an in situ photopolymerized thermoresponsive hydrogel nanoparticle system using human aortic smooth muscle cells Sabnis, Abhimanyu; Rahimi, Maham; Chapman, Christopher Journal of Biomedical Materials Research Part A, Vol. 91A, Issue 1 https://doi.org/10.1002/jbm.a.32194	journal	October 2009
Bioprinting for cancer research Knowlton, Stephanie; Onal, Sevgi; Yu, Chu Hsiang Trends in Biotechnology, Vol. 33, Issue 9 https://doi.org/10.1016/j.tibtech.2015.06.007	journal	September 2015
Cellulose Nanocrystal Inks for 3D Printing of Textured Cellular Architectures Siqueira, Gilberto; Kokkinis, Dimitri; Libanori, Rafael Advanced Functional Materials, Vol. 27, Issue 12 https://doi.org/10.1002/adfm.201604619	journal	February 2017
Photo-crosslinked poly(ethylene glycol) diacrylate (PEGDA) hydrogels from low molecular weight prepolymer: Swelling and permeation studies: ARTICLE Cavallo, Anna; Madaghiele, Marta; Masullo, Ugo Journal of Applied Polymer Science, Vol. 134, Issue 2 https://doi.org/10.1002/app.44380	journal	September 2016
Three-dimensional bioprinting of thick vascularized tissues Kolesky, David B.; Homan, Kimberly A.; Skylar-Scott, Mark A. Proceedings of the National Academy of Sciences, Vol. 113, Issue 12 https://doi.org/10.1073/pnas.1521342113	journal	March 2016
Responsive Materials: 3D Printing of Living Responsive Materials and Devices (Adv. Mater. 4/2018) Liu, Xinyue; Yuk, Hyunwoo; Lin, Shaoting Advanced Materials, Vol. 30, Issue 4 https://doi.org/10.1002/adma.201870021	journal	January 2018
Optimization of Culture Medium for the Growth of Candida sp. and Blastobotrys sp. as Starter Culture in Fermentation of Cocoa Beans (Theobroma cacao) Using Response Surface Methodology (RSM) Mahazar, N. H.; Zakuan, Z.; Norhayati, H. Pakistan Journal of Biological Sciences, Vol. 20, Issue 3 https://doi.org/10.3923/pjbs.2017.154.159	journal	February 2017
Direct‐Write Assembly of Microperiodic Silk Fibroin Scaffolds for Tissue Engineering Applications Ghosh, Sourabh; Parker, Sara T.; Wang, Xianyan Advanced Functional Materials, Vol. 18, Issue 13 https://doi.org/10.1002/adfm.200800040	journal	July 2008
Metabolically Engineered Yeasts: ‘Potential’ Industrial Applications Branduardi, Paola; Smeraldi, Carla; Porro, Danilo Journal of Molecular Microbiology and Biotechnology, Vol. 15, Issue 1 https://doi.org/10.1159/000111990	journal	January 2008
Additive Manufacturing of Catalytically Active Living Materials Saha, Abhijit; Johnston, Trevor G.; Shafranek, Ryan T. ACS Applied Materials & Interfaces, Vol. 10, Issue 16 https://doi.org/10.1021/acsami.8b02719	journal	April 2018
A Straightforward Approach for 3D Bacterial Printing Lehner, Benjamin A. E.; Schmieden, Dominik T.; Meyer, Anne S. ACS Synthetic Biology, Vol. 6, Issue 7 https://doi.org/10.1021/acssynbio.6b00395	journal	March 2017

Figures / Tables (5)

Similar Records

Self Assembly-Assisted Additive Manufacturing: Direct Ink Write 3D Printing of Epoxy-Amine Thermosets

Journal Article · Tue Dec 04 00:00:00 EST 2018 · Macromolecular Materials and Engineering · OSTI ID:1495251

Manning, Kylie B.; Wyatt, Nicholas B.; Hughes, Lindsey Gloe; +5 more

A Dual Approach in Direct Ink Writing of Thermally Cured Shape Memory Rubber Toughened Epoxy

Journal Article · Wed Nov 18 00:00:00 EST 2020 · ACS Applied Polymer Materials · OSTI ID:1495251

Chen, Qiyi; Sukmanee, Thanyada; Rong, Lihan; +4 more

Balancing Functionality and Printability: High-Loading Polymer Resins for Direct Ink Writing

Journal Article · Tue Nov 01 00:00:00 EDT 2022 · Polymers · OSTI ID:1495251

Legett, Shelbie A.; Torres, Xavier; Schmalzer, Andrew M.; +5 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
additive manufacturing
biocatalysts
bioinks
bioprinting
living materials
36 MATERIALS SCIENCE

Title: Direct Writing of Tunable Living Inks for Bioprocess Intensification

Citation Formats

References (25)

Figures / Tables (5)

Similar Records

Related Subjects