Photoheterotrophic growth of Physcomitrella patens

Bricker, Terry M.; Bell, Adam J.; Tran, Lan; Frankel, Laurie K.; Theg, Steven M.

doi:10.1007/s00425-013-2000-3

Photoheterotrophic growth of Physcomitrella patens

Journal Article · Tue Nov 26 23:00:00 EST 2013 · Planta

DOI:https://doi.org/10.1007/s00425-013-2000-3· OSTI ID:1490434

Bricker, Terry M. ^[1]; Bell, Adam J. ^[2]; Tran, Lan ^[3]; Frankel, Laurie K. ^[2]; Theg, Steven M. ^[3]

Louisiana State Univ., Baton Rouge, LA (United States); University of California - Davis
Louisiana State Univ., Baton Rouge, LA (United States)
Univ. of California, Davis, CA (United States)

Physcomitrella patens is a model bryophyte representing an early land plant in the green plant lineage. This organism possesses many advantages as a model organism. Its genome has been sequenced, its predominant life cycle stage is the haploid gametophyte, it is readily transformable and it can integrate transformed DNA into its genome by homologous recombination. One limitation for the use of P. patens in photosynthesis research is its reported inability to grow photoheterotrophically, in the presence of sucrose and the Photosystem II inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea, which prevents linear photosynthetic electron transport. In this communication we describe the facile isolation of a P. patens strain which can grow photoheterotrophically. Additionally, we have examined a number of photosynthetic parameters for this strain grown under photoautotrophic, mixotrophic (in the presence of sucrose) and photoheterotrophic conditions, as well as the 3-(3,4-dichlorophenyl)-1,1-dimethylurea-inhibited state. In conclusion, the ability to grow P. patens photoheterotrophically should significantly facilitate its use in photosynthetic studies.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: FG02-03ER15405

OSTI ID:: 1490434

Journal Information:: Planta, Journal Name: Planta Journal Issue: 3 Vol. 239; ISSN 0032-0935

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (30)

Functional analysis of the two homologous psbA gene copies in Synechocystis PCC 6714 and PCC 6803 Bouyoub, A.; Vernotte, C.; Astier, C. Plant Molecular Biology, Vol. 21, Issue 2 https://doi.org/10.1007/BF00019941	journal	January 1993
Sequencing and modification of psbB, the gene encoding the CP-47 protein of Photosystem II, in the cyanobacterium Synechocystis 6803 Vermaas, Wim F. J.; Williams, John G. K.; Arntzen, Charles J. Plant Molecular Biology, Vol. 8, Issue 4 https://doi.org/10.1007/BF00021311	journal	January 1987
Physcomitrella patens and Ceratodon purpureus, mosses as model organisms in photosynthesis studies Thornton, Leeann E.; Keren, Nir; Ohad, Itzhak Photosynthesis Research, Vol. 83, Issue 1 https://doi.org/10.1007/s11120-004-5577-3	journal	January 2005
Flash-yield pattern for photosynthetic oxygen evolution in Chlorella and chloroplasts as a function of excitation intensity Jursinic, Paul Archives of Biochemistry and Biophysics, Vol. 196, Issue 2 https://doi.org/10.1016/0003-9861(79)90300-X	journal	September 1979
[85] Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803 Williams, John G. K. Methods in Enzymology https://doi.org/10.1016/0076-6879(88)67088-1	book	January 1988
[22] Directed mutagenesis in photosystem II: Analysis of the CP 47 protein Bricker, Terry M.; Putnam-Evans, Cindy; Wu, Jituo Methods in Enzymology https://doi.org/10.1016/S0076-6879(98)97024-0	book	January 1998
The PsbP protein, but not the PsbQ protein, is required for normal thylakoid architecture in Arabidopsis thaliana Yi, Xiaoping; Hargett, Stefan R.; Frankel, Laurie K. FEBS Letters, Vol. 583, Issue 12 https://doi.org/10.1016/j.febslet.2009.05.048	journal	June 2009
Oxygen yield and thermoluminescence characteristics of a cyanobacterium lacking the manganese-stabilizing protein of photosystem II Burnap, Robert L.; Shen, J. R.; Jursinic, Paul A. Biochemistry, Vol. 31, Issue 32 https://doi.org/10.1021/bi00147a027	journal	August 1992
Efficient gene targeting in the moss Physcomitrella patens Schaefer, Didier G.; Zryd, Jean-Pierre The Plant Journal, Vol. 11, Issue 6 https://doi.org/10.1046/j.1365-313X.1997.11061195.x	journal	June 1997
Molecular Tools to Study Physcomitrella patens Frank, W.; Decker, E. L.; Reski, R. Plant Biology, Vol. 7, Issue 3 https://doi.org/10.1055/s-2005-865645	journal	May 2005
Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 C: Characterizations of two additional chlorophyll a-protein complexes Delepelaire, P.; Chua, N. -H. Proceedings of the National Academy of Sciences, Vol. 76, Issue 1 https://doi.org/10.1073/pnas.76.1.111	journal	January 1979
Herbicide resistance in Chlamydomonas reinhardtii results from a mutation in the chloroplast gene for the 32-kilodalton protein of photosystem II Erickson, J. M.; Rahire, M.; Bennoun, P. Proceedings of the National Academy of Sciences, Vol. 81, Issue 12 https://doi.org/10.1073/pnas.81.12.3617	journal	June 1984
Translational coupling of the maize chloroplast atpB and atpE genes Gatenby, A. A.; Rothstein, S. J.; Nomura, M. Proceedings of the National Academy of Sciences, Vol. 86, Issue 11 https://doi.org/10.1073/pnas.86.11.4066	journal	June 1989
Inactivation of the Chloroplast ATP Synthase γ Subunit Results in High Non-photochemical Fluorescence Quenching and Altered Nuclear Gene Expression in Arabidopsis thaliana Bosco, Cristina Dal; Lezhneva, Lina; Biehl, Alexander Journal of Biological Chemistry, Vol. 279, Issue 2 https://doi.org/10.1074/jbc.M308435200	journal	October 2003
Posttranslational events leading to the assembly of photosystem II protein complex: a study using photosynthesis mutants from Chlamydomonas reinhardtii de Vitry, C. The Journal of Cell Biology, Vol. 109, Issue 3 https://doi.org/10.1083/jcb.109.3.991	journal	September 1989
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS Arnon, Daniel I. Plant Physiology, Vol. 24, Issue 1 https://doi.org/10.1104/pp.24.1.1	journal	January 1949
Non-Mendelian Inheritance of 3-(3,4-Dichlorophenyl)-1,1-dimethylurea-Resistant Thylakoid Membrane Properties in Chlamydomonas Galloway, Ruth E.; Mets, Laurens Plant Physiology, Vol. 70, Issue 6 https://doi.org/10.1104/pp.70.6.1673	journal	December 1982
Evidence for Nuclear Control of the Expression of the atpA and atpB Chloroplast Genes in Chlamydomonas. Drapier, D.; Girard-Bascou, J.; Wollman, F. A. The Plant Cell https://doi.org/10.1105/tpc.4.3.283	journal	March 1992
Reduced Turnover of the D1 Polypeptide and Photoactivation of Electron Transfer in Novel Herbicide Resistant Mutants of Synechocystis sp. PCC 6803 Chjesa, Marta Dalla; Friso, Giulia; DeAK, Zsuzsanna European Journal of Biochemistry, Vol. 248, Issue 3 https://doi.org/10.1111/j.1432-1033.1997.00731.x	journal	September 1997
Molecular genetics of chloroplasts and mitochondria in the unicellular green alga Chlamydomonas Rochaix, J. -D. FEMS Microbiology Letters, Vol. 46, Issue 1 https://doi.org/10.1111/j.1574-6968.1987.tb02449.x	journal	March 1987
Un Nouveau Modele des Centres Photochimiques du Systeme ii Joliot, P.; Barbieri, G.; Chabaud, R. Photochemistry and Photobiology, Vol. 10, Issue 5 https://doi.org/10.1111/j.1751-1097.1969.tb05696.x	journal	November 1969
COOPERATION OF CHARGES IN PHOTOSYNTHETIC O ₂ EVOLUTION–I. A LINEAR FOUR STEP MECHANISM Kok, Bessel; Forbush, Bliss; McGLOIN, Marion Photochemistry and Photobiology, Vol. 11, Issue 6 https://doi.org/10.1111/j.1751-1097.1970.tb06017.x	journal	June 1970
Genetic and Physiological Studies of the Effect of Light on the Development of the Moss, Physcomitrella Patens Cove, D. J.; Schild, A.; Ashton, N. W. Photochemistry and Photobiology, Vol. 27, Issue 2 https://doi.org/10.1111/j.1751-1097.1978.tb07596.x	journal	February 1978
The Physcomitrella Genome Reveals Evolutionary Insights into the Conquest of Land by Plants Rensing, S. A.; Lang, D.; Zimmer, A. D. Science, Vol. 319, Issue 5859 https://doi.org/10.1126/science.1150646	journal	December 2007
Light-activated heterotrophic growth of the cyanobacterium Synechocystis sp. strain PCC 6803: a blue-light-requiring process. Anderson, S. L.; McIntosh, L. Journal of Bacteriology, Vol. 173, Issue 9 https://doi.org/10.1128/jb.173.9.2761-2767.1991	journal	May 1991
Mosses as Model Systems for the Study of Metabolism and Development Cove, David; Bezanilla, Magdalena; Harries, Phillip Annual Review of Plant Biology, Vol. 57, Issue 1 https://doi.org/10.1146/annurev.arplant.57.032905.105338	journal	June 2006
The Moss Physcomitrella patens Cove, David Annual Review of Genetics, Vol. 39, Issue 1 https://doi.org/10.1146/annurev.genet.39.073003.110214	journal	December 2005
Hepaticites devonicus A New Fossil Liverwort from the Devonian of New York Hueber, Francis M. Annals of the Missouri Botanical Garden, Vol. 48, Issue 2 https://doi.org/10.2307/2394879	journal	May 1961
Deletion Mutagenesis of the Cytochrome b 559 Protein Inactivates the Reaction Center of Photosystem II Pakrasi, Himadri B.; Diner, Bruce A.; Williams, John G. K. The Plant Cell, Vol. 1, Issue 6 https://doi.org/10.2307/3868946	journal	June 1989
Evolution of plant sucrose uptake transporters Reinders, Anke Frontiers in Plant Science, Vol. 3 https://doi.org/10.3389/fpls.2012.00022	journal	January 2012

Cited By (1)

Chloroplasts Require Glutathione Reductase to Balance Reactive Oxygen Species and Maintain Efficient Photosynthesis Müller-Schüssele, Stefanie J.; Wang, Ren; Gütle, Desirée D. The Plant Journal https://doi.org/10.1101/588442	posted_content	March 2019