Photoheterotrophic growth of Physcomitrella patens

Bricker, Terry M.; Bell, Adam J.; Tran, Lan; Frankel, Laurie K.; Theg, Steven M.

doi:10.1007/s00425-013-2000-3

Title: Photoheterotrophic growth of Physcomitrella patens

Journal Article · Wed Nov 27 00:00:00 EST 2013 · Planta

DOI:https://doi.org/10.1007/s00425-013-2000-3· OSTI ID:1490434

Bricker, Terry M. ^[1]; Bell, Adam J. ^[1]; Tran, Lan ^[2]; Frankel, Laurie K. ^[1]; Theg, Steven M. ^[2]

Louisiana State Univ., Baton Rouge, LA (United States)
Univ. of California, Davis, CA (United States)

Physcomitrella patens is a model bryophyte representing an early land plant in the green plant lineage. This organism possesses many advantages as a model organism. Its genome has been sequenced, its predominant life cycle stage is the haploid gametophyte, it is readily transformable and it can integrate transformed DNA into its genome by homologous recombination. One limitation for the use of P. patens in photosynthesis research is its reported inability to grow photoheterotrophically, in the presence of sucrose and the Photosystem II inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea, which prevents linear photosynthetic electron transport. In this communication we describe the facile isolation of a P. patens strain which can grow photoheterotrophically. Additionally, we have examined a number of photosynthetic parameters for this strain grown under photoautotrophic, mixotrophic (in the presence of sucrose) and photoheterotrophic conditions, as well as the 3-(3,4-dichlorophenyl)-1,1-dimethylurea-inhibited state. In conclusion, the ability to grow P. patens photoheterotrophically should significantly facilitate its use in photosynthetic studies.

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Research Organization:: Univ. of California, Davis, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

Grant/Contract Number:: FG02-03ER15405

OSTI ID:: 1490434

Journal Information:: Planta, Vol. 239, Issue 3; ISSN 0032-0935

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 3 works

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