Polymethine Thiopyrylium Fluorophores with Absorption beyond 1000 nm for Biological Imaging in the Second Near-infrared Sub-window

Ding, Bingbing; Xiao, Yuling; Zhou, Hui; Zhang, Xiao; Qu, Chunrong; Xu, Fuchun; Deng, Zixin; Cheng, Zhen; Hong, Xuechuan

doi:10.1021/acs.jmedchem.8b01682

Polymethine Thiopyrylium Fluorophores with Absorption beyond 1000 nm for Biological Imaging in the Second Near-infrared Sub-window

Journal Article · Sat Dec 01 23:00:00 EST 2018 · Journal of Medicinal Chemistry

DOI:https://doi.org/10.1021/acs.jmedchem.8b01682· OSTI ID:1485259

Ding, Bingbing ^[1]; Xiao, Yuling ^[2]; Zhou, Hui ^[2]; Zhang, Xiao ^[3]; Qu, Chunrong ^[3]; Xu, Fuchun ^[4]; Deng, Zixin ^[2]; Cheng, Zhen ^[3]; Hong, Xuechuan ^[5]

Wuhan Univ. School of Pharmaceutical Sciences, Wuhan (China); Stanford Univ., Stanford, CA (United States); Wuhan University School of Pharmaceutical Sciences
Wuhan Univ. School of Pharmaceutical Sciences, Wuhan (China)
Stanford Univ., Stanford, CA (United States)
Tibet Univ., Lasa (China)
Wuhan Univ. School of Pharmaceutical Sciences, Wuhan (China); Tibet Univ., Lasa (China); Shenzhen Institute of Wuhan Univ., Shenzhen (China)

Small-molecule fluorescence imaging in the second near-infrared (NIR-II, 1000-1700 nm) window has gained increasing interest in clinical application. Till now, very few studies have been exploited in the small-molecule fluorophores with both excitation and emission in the NIR-II window. Inspired by indocyanine green structure, a series of polymethine dyes with both absorption and emission in NIR-II window have been developed for NIR-II imaging, providing the feasibility to directly compare optical imaging in NIR-IIa (1300-1400 nm) sub-window under 1064 nm excitation with that in NIR-II window under 808 nm excitation. The signal-background ratio (SBR) and tumor to normal tissue ratio (T/NT) achieved great improvement under 1064 nm excitation in the imaging of mouse blood pool and U87 glioma tumors. Furthermore, our study not only introduces a broadband emission fluorophore for both NIR-II and NIR-IIa imaging, but also reveals the advantages of NIR-II excitation over NIR-I in in vivo imaging.

View Accepted Manuscript (DOE)

Research Organization:: The Leland Stanford Junior Univ., Palo Alto, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0008397

OSTI ID:: 1485259

Journal Information:: Journal of Medicinal Chemistry, Journal Name: Journal of Medicinal Chemistry Journal Issue: 4 Vol. 62; ISSN 0022-2623

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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