A metallo-biopolymer conjugate of elastin-like polypeptide: photoluminescence enhancement in the coacervate microenvironment

Ghosh, Koushik; Elbert, Katherine C.; Balog, Eva Rose M.; Martinez, Jennifer S.; Rocha, Reginaldo C.

doi:10.1007/s00775-018-1580-6

Title: A metallo-biopolymer conjugate of elastin-like polypeptide: photoluminescence enhancement in the coacervate microenvironment

Journal Article · Fri Jun 22 00:00:00 EDT 2018 · JBIC Journal of Biological Inorganic Chemistry

DOI:https://doi.org/10.1007/s00775-018-1580-6· OSTI ID:1482928

Ghosh, Koushik ^[1]; Elbert, Katherine C. ^[1]; Balog, Eva Rose M. ^[1]; Martinez, Jennifer S. ^[1]; Rocha, Reginaldo C. ^[1]

Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

An optically active metallo-polymer assembly is demonstrated here via conjugation of a genetically engineered elastin-like polypeptide (ELP) and a ruthenium(II) polypyridyl complex. By taking advantage of the phase transition of ELPs in water, photophysical properties of the resultant conjugate are investigated for both phases, below and above the critical transition temperature. Upon coacervation, the luminescence of the metallo-ELP is greatly enhanced as a consequence of local effects on the metal–ligand luminophore. Finally, these findings open a possibility to harness the temperature control of stimuli-responsive properties of biopolymers.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Workforce Development for Teachers and Scientists (WDTS); USDOE National Nuclear Security Administration (NNSA); LANL Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1482928

Report Number(s):: LA-UR-18-21561

Journal Information:: JBIC Journal of Biological Inorganic Chemistry, Vol. 23, Issue 7; ISSN 0949-8257

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 3 works

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References (29)

An Underwater Surface-Drying Peptide Inspired by a Mussel Adhesive Protein Wei, Wei; Petrone, Luigi; Tan, YerPeng Advanced Functional Materials, Vol. 26, Issue 20 https://doi.org/10.1002/adfm.201600210	journal	March 2016
Infiltration of chitin by protein coacervates defines the squid beak mechanical gradient Tan, YerPeng; Hoon, Shawn; Guerette, Paul A. Nature Chemical Biology, Vol. 11, Issue 7 https://doi.org/10.1038/nchembio.1833	journal	June 2015
Coacervation of tropoelastin Yeo, Giselle C.; Keeley, Fred W.; Weiss, Anthony S. Advances in Colloid and Interface Science, Vol. 167, Issue 1-2 https://doi.org/10.1016/j.cis.2010.10.003	journal	September 2011
Phase separation drives heterochromatin domain formation Strom, Amy R.; Emelyanov, Alexander V.; Mir, Mustafa Nature, Vol. 547, Issue 7662 https://doi.org/10.1038/nature22989	journal	June 2017
Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation Brangwynne, C. P.; Eckmann, C. R.; Courson, D. S. Science, Vol. 324, Issue 5935 https://doi.org/10.1126/science.1172046	journal	May 2009
Supramolecular control over thermoresponsive polymers de la Rosa, Victor R.; Woisel, Patrice; Hoogenboom, Richard Materials Today, Vol. 19, Issue 1 https://doi.org/10.1016/j.mattod.2015.06.013	journal	January 2016
Metallo-Biopolymers: Conjugation Strategies and Applications Sista, Prakash; Ghosh, Koushik; Martinez, Jennifer S. Polymer Reviews, Vol. 54, Issue 4 https://doi.org/10.1080/15583724.2014.913063	journal	August 2014
Stimuli-reponsive polymers and their bioconjugates Gil, E.; Hudson, S. Progress in Polymer Science, Vol. 29, Issue 12 https://doi.org/10.1016/j.progpolymsci.2004.08.003	journal	December 2004
Stimulus responsive elastin biopolymers: applications in medicine and biotechnology Chilkoti, A.; Christensen, T.; Mackay, J. Current Opinion in Chemical Biology, Vol. 10, Issue 6 https://doi.org/10.1016/j.cbpa.2006.10.010	journal	December 2006
Emerging applications of stimuli-responsive polymer materials Stuart, Martien A. Cohen; Huck, Wilhelm T. S.; Genzer, Jan Nature Materials, Vol. 9, Issue 2, p. 101-113 https://doi.org/10.1038/nmat2614	journal	January 2010
Programming molecular self-assembly of intrinsically disordered proteins containing sequences of low complexity Simon, Joseph R.; Carroll, Nick J.; Rubinstein, Michael Nature Chemistry, Vol. 9, Issue 6 https://doi.org/10.1038/nchem.2715	journal	January 2017
Genetically Encoded Synthesis of Protein-Based Polymers with Precisely Specified Molecular Weight and Sequence by Recursive Directional Ligation: Examples from the Elastin-like Polypeptide System Meyer, Dan E.; Chilkoti, Ashutosh Biomacromolecules, Vol. 3, Issue 2 https://doi.org/10.1021/bm015630n	journal	March 2002
Strong, Tough, Stretchable, and Self-Adhesive Hydrogels from Intrinsically Unstructured Proteins Gonzalez, Mark A.; Simon, Joseph R.; Ghoorchian, Ali Advanced Materials, Vol. 29, Issue 10 https://doi.org/10.1002/adma.201604743	journal	January 2017
Phase-structure transitions of the elastin polypentapeptide-water system within the framework of composition-temperature studies Urry, D. W.; Trapane, T. L.; Prasad, K. U. Biopolymers, Vol. 24, Issue 12 https://doi.org/10.1002/bip.360241212	journal	December 1985
Elastin-like polypeptides: Biomedical applications of tunable biopolymers MacEwan, Sarah R.; Chilkoti, Ashutosh Biopolymers, Vol. 94, Issue 1 https://doi.org/10.1002/bip.21327	journal	January 2010
Recombinant human elastin polypeptides self-assemble into biomaterials with elastin-like properties Bellingham, Catherine M.; Lillie, Margo A.; Gosline, John M. Biopolymers, Vol. 70, Issue 4 https://doi.org/10.1002/bip.10512	journal	November 2003
Elastin-based materials Almine, Jessica F.; Bax, Daniel V.; Mithieux, Suzanne M. Chemical Society Reviews, Vol. 39, Issue 9 https://doi.org/10.1039/b919452p	journal	January 2010
Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application Floss, Doreen M.; Schallau, Kai; Rose-John, Stefan Trends in Biotechnology, Vol. 28, Issue 1 https://doi.org/10.1016/j.tibtech.2009.10.004	journal	January 2010
Applications of luminescent transition platinum group metal complexes to sensor technology and molecular probes Demas, J. N.; DeGraff, B. A. Coordination Chemistry Reviews, Vol. 211, Issue 1 https://doi.org/10.1016/S0010-8545(00)00278-2	journal	January 2001
A ruthenium(II) polypyridyl complex for direct imaging of DNA structure in living cells Gill, Martin R.; Garcia-Lara, Jorge; Foster, Simon J. Nature Chemistry, Vol. 1, Issue 8 https://doi.org/10.1038/nchem.406	journal	October 2009
Medium Effects on Charge Transfer in Metal Complexes Chen, Pingyun; Meyer, Thomas J. Chemical Reviews, Vol. 98, Issue 4 https://doi.org/10.1021/cr941180w	journal	June 1998
Multicolor Luminescence from Conjugates of Genetically Encoded Elastin-like Polymers and Terpyridine-Lanthanides Ghosh, Koushik; Balog, Eva Rose M.; Kahn, Jennifer L. Macromolecular Chemistry and Physics, Vol. 216, Issue 18 https://doi.org/10.1002/macp.201500103	journal	August 2015
Temperature-dependent morphology of hybrid nanoflowers from elastin-like polypeptides Ghosh, Koushik; Balog, Eva Rose M.; Sista, Prakash APL Materials, Vol. 2, Issue 2 https://doi.org/10.1063/1.4863235	journal	February 2014
Oxidative electropolymerization of polypyridyl complexes of ruthenium Ellis, Charles D.; Margerum, Lawrence D.; Murray, Royce W. Inorganic Chemistry, Vol. 22, Issue 9 https://doi.org/10.1021/ic00151a005	journal	April 1983
Ru(II) polypyridine complexes: photophysics, photochemistry, eletrochemistry, and chemiluminescence Juris, A.; Balzani, V.; Barigelletti, F. Coordination Chemistry Reviews, Vol. 84 https://doi.org/10.1016/0010-8545(88)80032-8	journal	March 1988
Environmental Sensitivity of Ru(II) Complexes: The Role of the Accessory Ligands Dixon, Eileen N.; Snow, Michael Z.; Bon, Jennifer L. Inorganic Chemistry, Vol. 51, Issue 6 https://doi.org/10.1021/ic201114u	journal	March 2012
A Highly Selective OFF-ON Red-Emitting Phosphorescent Thiol Probe with Large Stokes Shift and Long Luminescent Lifetime Ji, Shaomin; Guo, Huimin; Yuan, Xiaolin Organic Letters, Vol. 12, Issue 12 https://doi.org/10.1021/ol100999j	journal	June 2010
The Luminescence Rigidochromic Effect Exhibited by Organometallic Complexes: Rationale and Applications Lees, Alistair J. Comments on Inorganic Chemistry, Vol. 17, Issue 6 https://doi.org/10.1080/02603599508032711	journal	November 1995
Protein- and peptide-modified synthetic polymeric biomaterials Krishna, Ohm D.; Kiick, Kristi L. Biopolymers, Vol. 94, Issue 1 https://doi.org/10.1002/bip.21333	journal	January 2010

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Macro- and Microphase Separated Protein-Polyelectrolyte Complexes: Design Parameters and Current Progress Horn, Justin; Kapelner, Rachel; Obermeyer, Allie Polymers, Vol. 11, Issue 4 https://doi.org/10.3390/polym11040578	journal	March 2019

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