The mobility of packaged phage genome controls ejection dynamics

Evilevitch, Alex

doi:10.7554/eLife.37345

The mobility of packaged phage genome controls ejection dynamics

Journal Article · Tue Sep 04 04:00:00 EDT 2018 · eLife

DOI:https://doi.org/10.7554/eLife.37345· OSTI ID:1478089

^[1]

Department of Pathobiology, Division of Microbiology and Immunology, College of Veterinary Medicine, University of Illinois at Urbana-Champaign, Champaign, United States; Department of Experimental Medical Sciences, Virus Biophysics Group, Lund University, Lund, Sweden

The cell decision between lytic and lysogenic infection is strongly influenced by dynamics of DNA injection into a cell from a phage population, as phages compete for limited resources and progeny. However, what controls the timing of viral DNA ejection events was not understood. This in vitro study reveals that DNA ejection dynamics for phages can be synchronized (occurring within seconds) or desynchronized (displaying minutes-long delays in initiation) based on mobility of encapsidated DNA, which in turn is regulated by environmental factors, such as temperature and extra-cellular ionic conditions. This mechano-regulation of ejection dynamics is suggested to influence viral replication where the cell’s decision between lytic and latent infection is associated with synchronized or desynchronized delayed ejection events from phage population adsorbed to a cell. Our findings are of significant importance for understanding regulatory mechanisms of latency in phage and Herpesviruses, where encapsidated DNA undergoes a similar mechanical transition.

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: NSFOTHER

OSTI ID:: 1478089

Journal Information:: eLife, Journal Name: eLife Journal Issue: 2018 Vol. 7; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.

Country of Publication:: United States

Language:: ENGLISH

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