Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser

Nogly, Przemyslaw; Weinert, Tobias; James, Daniel; Carbajo, Sergio; Ozerov, Dmitry; Furrer, Antonia; Gashi, Dardan; Borin, Veniamin; Skopintsev, Petr; Jaeger, Kathrin; Nass, Karol; Båth, Petra; Bosman, Robert; Koglin, Jason; Seaberg, Matthew; Lane, Thomas; Kekilli, Demet; Brünle, Steffen; Tanaka, Tomoyuki; Wu, Wenting; Milne, Christopher; White, Thomas; Barty, Anton; Weierstall, Uwe; Panneels, Valerie; Nango, Eriko; Iwata, So; Hunter, Mark; Schapiro, Igor; Schertler, Gebhard; Neutze, Richard; Standfuss, Jörg

doi:10.1126/science.aat0094

Retinal isomerization in bacteriorhodopsin captured by a femtosecond x-ray laser

Journal Article · Fri Jul 13 00:00:00 EDT 2018 · Science

DOI:https://doi.org/10.1126/science.aat0094· OSTI ID:1470908

^[1]; ^[2]; ^[1]; ^[3]; Ozerov, Dmitry ^[4]; ^[1]; Gashi, Dardan ^[5]; ^[6]; Skopintsev, Petr ^[1]; Jaeger, Kathrin ^[1]; ^[7]; Båth, Petra ^[8]; Bosman, Robert ^[8]; ^[3]; ^[3]; ^[3]; Kekilli, Demet ^[1]; Brünle, Steffen ^[1]; Tanaka, Tomoyuki ^[9]; Wu, Wenting ^[1] more »

Paul Scherrer Institut (Switzerland). Division of Biology and Chemistry–Laboratory for Biomolecular Research
Paul Scherrer Institut (Switzerland). Division of Biology and Chemistry–Laboratory for Biomolecular Research and Photon Science Division–Swiss Light Source
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Paul Scherrer Institut (Switzerland). Science IT
Paul Scherrer Institut (Switzerland). SwissFEL
The Hebrew University of Jerusalem (Israel). Fritz Haber Center for Molecular Dynamics, Institute of Chemistry
Paul Scherrer Institut (Switzerland). SwissFEL and Photon Science Division–Swiss Light Source
University of Gothenburg (Sweden). Department of Chemistry and Molecular Biology
RIKEN SPring-8 Center (Japan); Kyoto University (Japan). Department of Cell Biology, Graduate School of Medicine
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany).Center for Free-Electron Laser Science (CFEL)
Arizona State Univ., Tempe, AZ (United States). Department of Physics
Paul Scherrer Institut (Switzerland). Division of Biology and Chemistry–Laboratory for Biomolecular Research ; ETH Zürich (Switzerland). Department of Biology

Ultrafast isomerization of retinal is the primary step in photoresponsive biological functions including vision in humans and ion transport across bacterial membranes. In this study, we used an x-ray laser to study the subpicosecond structural dynamics of retinal isomerization in the light-driven proton pump bacteriorhodopsin. A series of structural snapshots with near-atomic spatial resolution and temporal resolution in the femtosecond regime show how the excited all-trans retinal samples conformational states within the protein binding pocket before passing through a twisted geometry and emerging in the 13-cis conformation. In conclusion, our findings suggest ultrafast collective motions of aspartic acid residues and functional water molecules in the proximity of the retinal Schiff base as a key facet of this stereoselective and efficient photochemical reaction.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1470908

Journal Information:: Science, Journal Name: Science Journal Issue: 6398 Vol. 361; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017953	journal	February 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
Well-based crystallization of lipidic cubic phase microcrystals for serial X-ray crystallography experiments Andersson, Rebecka; Safari, Cecilia; Båth, Petra Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 10 https://doi.org/10.1107/s2059798319012695	journal	October 2019
Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography Weinert, Tobias; Skopintsev, Petr; James, Daniel Science, Vol. 365, Issue 6448 https://doi.org/10.1126/science.aaw8634	journal	July 2019
Collective exchange processes reveal an active site proton cage in bacteriorhodopsin Friedrich, Daniel; Brünig, Florian N.; Nieuwkoop, Andrew J. Freie Universität Berlin https://doi.org/10.17169/refubium-26480	text	January 2020
Biological single-particle imaging using XFELs – towards the next resolution revolution Oberthür, Dominik Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2018-04825	text	January 2018
Time-resolved serial femtosecond crystallography at the European XFEL Pandey, Suraj; Bean, Richard; Sato, Tokushi Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-04293	text	January 2019
Ultracompact 3D microfluidics for time-resolved structural biology Knoska, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00623	text	January 2020
Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00687	text	January 2020
Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin Kovacs, Gabriela Nass; Colletier, Jacques-Philippe; Grünbein, Marie arXiv https://doi.org/10.48550/arxiv.1905.09002	text	January 2019

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