A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme

Mirts, Evan N.; Petrik, Igor D.; Hosseinzadeh, Parisa; Nilges, Mark J.; Lu, Yi

doi:10.1126/science.aat8474

Title: A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme

Journal Article · Fri Sep 14 00:00:00 EDT 2018 · Science

DOI:https://doi.org/10.1126/science.aat8474· OSTI ID:1470758

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^[2]; Nilges, Mark J. ^[3];

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Univ. of Illinois at Urbana-Champaign, IL (United States). Center for Biophysics and Quantitative Biology
Univ. of Illinois at Urbana-Champaign, IL (United States)
Univ. of Illinois at Urbana-Champaign, IL (United States). School of Chemical Sciences Electron Paramagnetic Resonance Lab.
Univ. of Illinois at Urbana-Champaign, IL (United States). Center for Biophysics and Quantitative Biology, Dept. of Chemistry, Dept. of Biochemistry, Carl R. Woese Inst. for Genomic Biology and Center for Advanced Bioenergy and Bioproducts Innovation (CABBI); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Metals brought together do more Enzymatic reduction of oxyanions such as sulfite (SO 3 2− ) requires the delivery of multiple electrons and protons, a feat accomplished by cofactors tailored for catalysis and electron transport. Replicating this strategy in protein scaffolds may expand the range of enzymes that can be designed de novo. Mirts et al. selected a scaffold protein containing a natural heme cofactor and then engineered a cavity suitable for binding a second cofactor—an iron-sulfur cluster (see the Perspective by Lancaster). The resulting designed enzyme was optimized through rational mutation into a catalyst with spectral characteristics and activity similar to that of natural sulfite reductases. Science , this issue p. 1098 ; see also p. 1071

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Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS)

Contributing Organization:: SLAC National Accelerator Laboratory (SLAC); Karolinska Institute

Grant/Contract Number:: SC0018420; R01-GM062211; 5T32-GM827625; AC02-76SF00515

OSTI ID:: 1470758

Alternate ID(s):: OSTI ID: 1991812

Journal Information:: Science, Vol. 361, Issue 6407; ISSN 0036-8075

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 84 works

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Title: A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme

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