Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer

Stadnytskyi, Valentyn; Orf, Gregory S.; Blankenship, Robert E.; Savikhin, Sergei

doi:10.1063/1.5009468

Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer

Journal Article · Mon Mar 12 00:00:00 EDT 2018 · Review of Scientific Instruments

DOI:https://doi.org/10.1063/1.5009468· OSTI ID:1470599

^[1]; ^[2]; Blankenship, Robert E. ^[2]; Savikhin, Sergei ^[1]

Purdue Univ., West Lafayette, IN (United States). Dept. of Physics and Astronomy
Washington Univ., St. Louis, MO (United States). Dept. of Biology and Chemistry, Photosynthetic Antenna Research Center

We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of $$\mu$$deg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. Finally, the spectrometer was used to record, for the first time, weak TRCD kinetics associated with the triplet state energy transfer in the photosynthetic Fenna-Matthews-Olson antenna pigment-protein complex.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0001035; SC0001341

OSTI ID:: 1470599

Alternate ID(s):: OSTI ID: 1425540

Journal Information:: Review of Scientific Instruments, Journal Name: Review of Scientific Instruments Journal Issue: 3 Vol. 89; ISSN 0034-6748

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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