Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer

Stadnytskyi, Valentyn; Orf, Gregory S.; Blankenship, Robert E.; Savikhin, Sergei

doi:10.1063/1.5009468

Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer

Journal Article · Mon Mar 12 00:00:00 EDT 2018 · Review of Scientific Instruments

DOI:https://doi.org/10.1063/1.5009468· OSTI ID:1470599

^[1]; ^[2]; Blankenship, Robert E. ^[2]; Savikhin, Sergei ^[1]

Purdue Univ., West Lafayette, IN (United States). Dept. of Physics and Astronomy
Washington Univ., St. Louis, MO (United States). Dept. of Biology and Chemistry, Photosynthetic Antenna Research Center

We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of $$\mu$$deg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. Finally, the spectrometer was used to record, for the first time, weak TRCD kinetics associated with the triplet state energy transfer in the photosynthetic Fenna-Matthews-Olson antenna pigment-protein complex.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0001035

OSTI ID:: 1470599

Alternate ID(s):: OSTI ID: 1425540

Journal Information:: Review of Scientific Instruments, Journal Name: Review of Scientific Instruments Journal Issue: 3 Vol. 89; ISSN 0034-6748

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

References (51)

Stopped-flow circular dichroism: A new fast-kinetic system Bayley, P. M.; Anson, M. Biopolymers, Vol. 13, Issue 2 https://doi.org/10.1002/bip.1974.360130215	journal	February 1974
Circular Dichroism and the Conformational Analysis of Biomolecules Fasman, Gerald D. https://doi.org/10.1007/978-1-4757-2508-7	book	January 1996
Recent advances in time resolved circular dichroism spectroscopy Kliger, D. S.; Lewis, J. W. Reviews of Chemical Intermediates, Vol. 8, Issue 4 https://doi.org/10.1007/bf03055509	journal	October 1987
Linear dichroism and circular dichroism in photosynthesis research Garab, Győző; van Amerongen, Herbert Photosynthesis Research, Vol. 101, Issue 2-3 https://doi.org/10.1007/s11120-009-9424-4	journal	May 2009
Revisiting the optical properties of the FMO protein Milder, Maaike T. W.; Brüggemann, Ben; van Grondelle, Rienk Photosynthesis Research, Vol. 104, Issue 2-3 https://doi.org/10.1007/s11120-010-9540-1	journal	March 2010
Ultrasensitive time-resolved linear dichroism spectral measurements using near-crossed linear polarizers Che, Diping; Shapiro, Daniel B.; Esquerra, Raymond M. Chemical Physics Letters, Vol. 224, Issue 1-2 https://doi.org/10.1016/0009-2614(94)00530-3	journal	July 1994
Perturbation of bacteriochlorophyll molecules in Fenna–Matthews–Olson protein complexes through mutagenesis of cysteine residues Saer, Rafael; Orf, Gregory S.; Lu, Xun Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1857, Issue 9 https://doi.org/10.1016/j.bbabio.2016.04.007	journal	September 2016
How to study proteins by circular dichroism Kelly, Sharon M.; Jess, Thomas J.; Price, Nicholas C. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1751, Issue 2 https://doi.org/10.1016/j.bbapap.2005.06.005	journal	August 2005
Application of time-resolved circular dichroism to the study of conformational changes in photochemical and photobiological processes Hache, François Journal of Photochemistry and Photobiology A: Chemistry, Vol. 204, Issue 2-3 https://doi.org/10.1016/j.jphotochem.2009.03.012	journal	May 2009
Quantum coherence in photosynthesis Engel, Gregory S. Procedia Chemistry, Vol. 3, Issue 1 https://doi.org/10.1016/j.proche.2011.08.029	journal	January 2011
Nanosecond time-resolved polarization spectroscopies: Tools for probing protein reaction mechanisms Chen, Eefei; Goldbeck, Robert A.; Kliger, David S. Methods, Vol. 52, Issue 1 https://doi.org/10.1016/j.ymeth.2010.04.015	journal	September 2010
A picosecond circular dichroism study of photosynthetic reaction centers from Rhodobacter sphaeroides Xie, Xiaoliang; Simon, John D. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1057, Issue 1 https://doi.org/10.1016/s0005-2728(05)80093-3	journal	March 1991
Time-resolved circular dichroism and absorption studies of the photolysis reaction of (carbonmonoxy)myoglobin Milder, S. J.; Bjorling, S. C.; Kuntz, I. D. Biophysical Journal, Vol. 53, Issue 5 https://doi.org/10.1016/s0006-3495(88)83146-1	journal	May 1988
Nanosecond optical rotatory dispersion spectroscopy: application to photolyzed hemoglobin-CO kinetics Shapiro, D. B.; Goldbeck, R. A.; Che, D. Biophysical Journal, Vol. 68, Issue 1 https://doi.org/10.1016/s0006-3495(95)80191-8	journal	January 1995
Oscillating anisotropies in a bacteriochlorophyll protein: Evidence for quantum beating between exciton levels Savikhin, Sergei; Buck, Daniel R.; Struve, Walter S. Chemical Physics, Vol. 223, Issue 2-3 https://doi.org/10.1016/s0301-0104(97)00223-1	journal	November 1997
Effect of Spectral Density Shapes on the Excitonic Structure and Dynamics of the Fenna–Matthews–Olson Trimer from Chlorobaculum tepidum Kell, Adam; Blankenship, Robert E.; Jankowiak, Ryszard The Journal of Physical Chemistry A, Vol. 120, Issue 31 https://doi.org/10.1021/acs.jpca.6b03107	journal	August 2016
Allosteric Intermediates in Hemoglobin. 1. Nanosecond Time-Resolved Circular Dichroism Spectroscopy ^† Björling, Sofie C.; Goldbeck, Robert A.; Paquette, Sarah J. Biochemistry, Vol. 35, Issue 26 https://doi.org/10.1021/bi952247s	journal	January 1996
Nanosecond circular dichroism spectral measurements: extension to the far-ultraviolet region Zhang, C. F.; Lewis, J. W.; Cerpa, R. The Journal of Physical Chemistry, Vol. 97, Issue 21 https://doi.org/10.1021/j100123a009	journal	May 1993
Analysis of optical artifacts in ellipsometric measurements of time-resolved circular dichroism Bjorling, Sofie C.; Goldbeck, Robert A.; Milder, Steven J. The Journal of Physical Chemistry, Vol. 95, Issue 12 https://doi.org/10.1021/j100165a018	journal	June 1991
New technique for measuring circular dichroism changes on a nanosecond time scale. Application to (carbonmonoxy)myoglobin and (carbonmonoxy)hemoglobin Lewis, J. W.; Tilton, R. F.; Einterz, C. M. The Journal of Physical Chemistry, Vol. 89, Issue 2 https://doi.org/10.1021/j100248a023	journal	January 1985
Birefringence effects in transient circular dichroism measurements with applications to the photolysis of carbon monoxyhemoglobin and carbon monoxymyoglobin Einterz, C. M.; Lewis, J. W.; Milder, S. J. The Journal of Physical Chemistry, Vol. 89, Issue 18 https://doi.org/10.1021/j100264a015	journal	August 1985
Time-resolved circular dichroism of native oat phytochrome photointermediates Bjorling, Sofie C.; Zhang, Chian Fan; Farrens, David L. Journal of the American Chemical Society, Vol. 114, Issue 12 https://doi.org/10.1021/ja00038a020	journal	June 1992
Ultrafast Carbonyl Motion of the Photoactive Yellow Protein Chromophore Probed by Femtosecond Circular Dichroism Mendonça, Lucille; Hache, François; Changenet-Barret, Pascale Journal of the American Chemical Society, Vol. 135, Issue 39 https://doi.org/10.1021/ja404503q	journal	September 2013
Conformational Changes in Photoexcited ( R )-(+)-1,1′-Bi-2-naphthol Studied by Time-Resolved Circular Dichroism Niezborala, Claire; Hache, François Journal of the American Chemical Society, Vol. 130, Issue 38 https://doi.org/10.1021/ja8039844	journal	September 2008
Exciton Analysis in 2D Electronic Spectroscopy Cho, Minhaeng; Vaswani, Harsha M.; Brixner, Tobias The Journal of Physical Chemistry B, Vol. 109, Issue 21 https://doi.org/10.1021/jp050788d	journal	June 2005
From Atomistic Modeling to Excitation Transfer and Two-Dimensional Spectra of the FMO Light-Harvesting Complex Olbrich, Carsten; Jansen, Thomas L. C.; Liebers, Jörg The Journal of Physical Chemistry B, Vol. 115, Issue 26 https://doi.org/10.1021/jp202619a	journal	July 2011
Intensity Dependence of the Excited State Lifetimes and Triplet Conversion Yield in the Fenna–Matthews–Olson Antenna Protein Orf, Gregory S.; Niedzwiedzki, Dariusz M.; Blankenship, Robert E. The Journal of Physical Chemistry B, Vol. 118, Issue 8 https://doi.org/10.1021/jp411020a	journal	February 2014
Comparative Study of the Folding/Unfolding Dynamics of Poly(glutamic acid) in Light and Heavy Water Mendonça, Lucille; Steinbacher, Andreas; Bouganne, Raphaël The Journal of Physical Chemistry B, Vol. 118, Issue 20 https://doi.org/10.1021/jp501282z	journal	May 2014
The Fate of the Triplet Excitations in the Fenna–Matthews–Olson Complex Kihara, Shigeharu; Hartzler, Daniel A.; Orf, Gregory S. The Journal of Physical Chemistry B, Vol. 119, Issue 18 https://doi.org/10.1021/jp512222c	journal	April 2015
Effect of Diagonal Energy Disorder on Circular Dichroism Spectra of Fenna−Matthews−Olson Trimers Buck, Daniel R.; Savikhin, Sergei; Struve, Walter S. The Journal of Physical Chemistry B, Vol. 101, Issue 42 https://doi.org/10.1021/jp971758g	journal	October 1997
Fast Time-Resolved Magnetic Optical Rotatory Dispersion Measurements. 1. Mueller Analysis of Optical and Photoselection-Induced Artifacts Esquerra, Raymond M.; Goldbeck, Robert A.; Kim-Shapiro, Daniel B. The Journal of Physical Chemistry A, Vol. 102, Issue 45 https://doi.org/10.1021/jp981693x	journal	November 1998
Exciton Simulations of Optical Spectra of the FMO Complex from the Green Sulfur Bacterium Chlorobium tepidum at 6 K Vulto, Simone I. E.; de Baat, Michiel A.; Louwe, Robert J. W. The Journal of Physical Chemistry B, Vol. 102, Issue 47 https://doi.org/10.1021/jp982095l	journal	November 1998
The FMO Protein Olson, John M. Photosynthesis Research, Vol. 80, Issue 1-3 https://doi.org/10.1023/b:pres.0000030428.36950.43	journal	January 2004
Chlorophyll arrangement in a bacteriochlorophyll protein from Chlorobium limicola Fenna, R. E.; Matthews, B. W. Nature, Vol. 258, Issue 5536 https://doi.org/10.1038/258573a0	journal	December 1975
Evidence for wavelike energy transfer through quantum coherence in photosynthetic systems Engel, Gregory S.; Calhoun, Tessa R.; Read, Elizabeth L. Nature, Vol. 446, Issue 7137 https://doi.org/10.1038/nature05678	journal	April 2007
Picosecond time‐resolved circular dichroism spectroscopy: experimental details and applications Xie, Xiaoliang; Simon, John D. Review of Scientific Instruments, Vol. 60, Issue 8 https://doi.org/10.1063/1.1140681	journal	August 1989
Nanosecond time‐resolved circular dichroism measurements using an upconverted Ti:sapphire laser Wen, You‐Xian; Chen, Eefei; Lewis, James W. Review of Scientific Instruments, Vol. 67, Issue 9 https://doi.org/10.1063/1.1147422	journal	September 1996
The measurement of transient circular dichroism: A new kinetic technique Ferrone, F. A.; Hopfield, J. J.; Schnatterly, S. E. Review of Scientific Instruments, Vol. 45, Issue 11 https://doi.org/10.1063/1.1686510	journal	November 1974
Broadband femtosecond circular dichroism spectrometer with white-light polarization control Trifonov, Anton; Buchvarov, Ivan; Lohr, Andreas Review of Scientific Instruments, Vol. 81, Issue 4 https://doi.org/10.1063/1.3340892	journal	April 2010
Communication: Broadband and ultrasensitive femtosecond time-resolved circular dichroism spectroscopy Hiramatsu, Kotaro; Nagata, Takashi The Journal of Chemical Physics, Vol. 143, Issue 12 https://doi.org/10.1063/1.4932229	journal	September 2015
Evidence for a cysteine-mediated mechanism of excitation energy regulation in a photosynthetic antenna complex Orf, Gregory S.; Saer, Rafael G.; Niedzwiedzki, Dariusz M. Proceedings of the National Academy of Sciences, Vol. 113, Issue 31 https://doi.org/10.1073/pnas.1603330113	journal	June 2016
Measurement of circular dichroism at millisecond time resolution: a stopped-flow circular dichroism system Anson, M.; Bayley, P. M. Journal of Physics E: Scientific Instruments, Vol. 7, Issue 6 https://doi.org/10.1088/0022-3735/7/6/024	journal	June 1974
Towards a coherent picture of excitonic coherence in the Fenna–Matthews–Olson complex Fidler, Andrew F.; Caram, Justin R.; Hayes, Dugan Journal of Physics B: Atomic, Molecular and Optical Physics, Vol. 45, Issue 15 https://doi.org/10.1088/0953-4075/45/15/154013	journal	July 2012
Circular Dichroism Techniques: Biomolecular and Nanostructural Analyses- A Review Ranjbar, Bijan; Gill, Pooria Chemical Biology & Drug Design, Vol. 74, Issue 2 https://doi.org/10.1111/j.1747-0285.2009.00847.x	journal	August 2009
Measuring the dynamics of circular dichroism in a pump-probe experiment with a Babinet-Soleil compensator: erratum Niezborala, Claire; Hache, François Journal of the Optical Society of America B, Vol. 24, Issue 4 https://doi.org/10.1364/josab.24.001012	journal	January 2007
Picosecond circular dichroism spectroscopy: a Jones matrix analysis Xie, Xiaoliang; Simon, John D. Journal of the Optical Society of America B, Vol. 7, Issue 8 https://doi.org/10.1364/josab.7.001673	journal	January 1990
Broadband near UV to visible optical activity measurement using self-heterodyned method Eom, Intae; Ahn, Sung-Hyun; Rhee, Hanju Optics Express, Vol. 19, Issue 10 https://doi.org/10.1364/oe.19.010017	journal	January 2011
Broadband transient dichroism spectroscopy in chiral molecules Mangot, Laureen; Taupier, Gregory; Romeo, Michelangelo Optics Letters, Vol. 35, Issue 3 https://doi.org/10.1364/ol.35.000381	journal	January 2010
How Proteins Trigger Excitation Energy Transfer in the FMO Complex of Green Sulfur Bacteria Adolphs, Julia; Renger, Thomas Biophysical Journal, Vol. 91, Issue 8 https://doi.org/10.1529/biophysj.105.079483	journal	October 2006
The Use of Circular Dichroism in the Investigation of Protein Structure and Function Kelly, Sharon; Price, Nicholas Current Protein & Peptide Science, Vol. 1, Issue 4, p. 349-384 https://doi.org/10.2174/1389203003381315	journal	December 2000
Probing Kinetic Mechanisms of Protein Function and Folding with Time-Resolved Natural and Magnetic Chiroptical Spectroscopies Kliger, David S.; Chen, Eefei; Goldbeck, Robert A. International Journal of Molecular Sciences, Vol. 13, Issue 1 https://doi.org/10.3390/ijms13010683	journal	January 2012

Cited By (2)

Ultrafast broadband circular dichroism in the deep ultraviolet Oppermann, Malte; Bauer, Benjamin; Rossi, Thomas Optica, Vol. 6, Issue 1 https://doi.org/10.1364/optica.6.000056	journal	January 2019
Ultrafast broadband circular dichroism in the deep ultraviolet Oppermann, Malte; Bauer, Benjamin; Rossi, Thomas Optical Society of America https://doi.org/10.5167/uzh-172268	text	January 2019

Similar Records

Revealing excitonic structure and charge transfer in photosynthetic proteins by time-resolved circular dichroism spectroscopy

Technical Report · Wed Nov 06 23:00:00 EST 2019 · OSTI ID:1509889

Monitoring aromatic ring-currents in Mg-porphyrin by time-resolved circular dichroism

Journal Article · Mon May 11 00:00:00 EDT 2020 · Physical Chemistry Chemical Physics. PCCP · OSTI ID:1849505

Related Subjects

47 OTHER INSTRUMENTATION
bio-inspired
biofuels (including algae and biomass)
charge transport
membrane
photosynthesis (natural and artificial)
solar (fuels)
synthesis (novel materials)
synthesis (self-assembly)

Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer

Citation Formats

References (51)

Cited By (2)

Similar Records

Related Subjects