Enhancing metabolic flux to photosynthetic biofuels
- Vanderbilt Univ., Nashville, TN (United States); Vanderbilt University
- Vanderbilt Univ., Nashville, TN (United States)
The overall objective of this project was to test new metabolic engineering and systems biology approaches for maximizing metabolic flux to biofuels in cyanobacterial hosts. Our central hypothesis was that combining directed metabolic engineering approaches with global rewiring of circadian transcriptional programs would lead to enhanced biofuel productivities. As proof of principle, we applied our novel toolset to optimize cyanobacterial production of isobutyraldehyde (IBA), which is a direct precursor of isobutanol, starting from a parental IBA-producing strain of Synechococcus elongatus PCC 7942.
- Research Organization:
- Vanderbilt Univ., Nashville, TN (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division
- DOE Contract Number:
- SC0008118
- OSTI ID:
- 1469067
- Report Number(s):
- DOE-VU--11111-1
- Country of Publication:
- United States
- Language:
- English
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