Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria

Choi, Sun Young; Lee, Hyun Jeong; Choi, Jaeyeon; Kim, Jiye; Sim, Sang Jun; Um, Youngsoon; Kim, Yunje; Lee, Taek Soon; Keasling, Jay D.; Woo, Han Min

doi:10.1186/s13068-016-0617-8

Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria

Journal Article · Thu Sep 22 00:00:00 EDT 2016 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-016-0617-8· OSTI ID:1626977

Choi, Sun Young ^[1]; Lee, Hyun Jeong ^[2]; Choi, Jaeyeon ^[2]; Kim, Jiye ^[3]; Sim, Sang Jun ^[4]; Um, Youngsoon ^[2]; Kim, Yunje ^[2]; Lee, Taek Soon ^[5]; Keasling, Jay D. ^[6]; ^[7]

Korea Inst. of Science and Technology, Seoul (Korea, Republic of). Clean Energy Research Center; Korea Univ., Seoul (Korea, Republic of). Green School. Graduate school of Energy and Environment; DOE/OSTI
Korea Inst. of Science and Technology, Seoul (Korea, Republic of). Clean Energy Research Center
Korea Inst. of Science and Technology, Seoul (Korea, Republic of). Clean Energy Research Center; Korea Univ., Seoul (Korea, Republic of). Dept. of Chemistry
Korea Univ., Seoul (Korea, Republic of). Green School. Graduate school of Energy and Environment; Korea Univ., Seoul (Korea, Republic of). Dept. of Chemical and Biological Engineering
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering; Univ. of California, Berkeley, CA (United States). Dept. of Chemical and Biomolecular Engineering
Sungkyunkwan Univ., Suwon (Republic of Korea). Dept. of Food Science and Biotechnology

Background: Metabolic engineering of cyanobacteria has enabled photosynthetic conversion of CO2 to value added chemicals as bio-solar cell factories. However, the production levels of isoprenoids in engineered cyanobacteria were quite low, compared to other microbial hosts. Therefore, modular optimization of multiple gene expressions for metabolic engineering of cyanobacteria is required for the production of farnesyl diphosphate-derived isoprenoids from CO2. Results: Here, we engineered Synechococcus elongatus PCC 7942 with modular metabolic pathways consisting of the methylerythritol phosphate pathway enzymes and the amorphadiene synthase for production of amorpha-4,11-diene, resulting in significantly increased levels (23-fold) of amorpha-4,11-diene (19.8 mg/L) in the best strain relative to a parental strain. Replacing amorphadiene synthase with squalene synthase led to the synthesis of a high amount of squalene (4.98 mg/L/OD730). Overexpression of farnesyl diphosphate synthase is the most critical factor for the significant production, whereas overexpression of 1-deoxy-d-xylulose 5-phosphate reductase is detrimental to the cell growth and the production. Additionally, the cyanobacterial growth inhibition was alleviated by expressing a terpene synthase in S. elongatus PCC 7942 strain with the optimized MEP pathway only (SeHL33). Conclusions: This is the first demonstration of photosynthetic production of amorpha-4,11-diene from CO2 in cyanobacteria and production of squalene in S. elongatus PCC 7942. Our optimized modular Over MEP strain (SeHL33) with either co-expression of ADS or SQS demonstrated the highest production levels of amorpha-4,11-diene and squalene, which could expand the list of farnesyl diphosphate-derived isoprenoids from CO2 as bio-solar cell factories.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1626977

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 9; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria Choi, Sun; Lee, Hyun; Choi, Jaeyeon https://doi.org/10.6084/m9.figshare.c.3625784 The current record is supplemented by this collection	collection	January 2016
Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria Choi, Sun; Lee, Hyun; Choi, Jaeyeon https://doi.org/10.6084/m9.figshare.c.3625784.v1 The current record is supplemented by this collection	collection	January 2016
MOESM1 of Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria Choi, Sun; Lee, Hyun; Choi, Jaeyeon https://doi.org/10.6084/m9.figshare.c.3625784_d1 The current record is supplemented by this text	text	January 2016
MOESM1 of Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria Choi, Sun; Lee, Hyun; Choi, Jaeyeon https://doi.org/10.6084/m9.figshare.c.3625784_d1.v1 The current record is supplemented by this text	text	January 2016

Photosynthetic conversion of CO2 to farnesyl diphosphate-derived phytochemicals (amorpha-4,11-diene and squalene) by engineered cyanobacteria

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