Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Lew, Tedrick Thomas Salim; Wong, Min Hao; Kwak, Seon-Yeong; Sinclair, Rosalie; Koman, Volodymyr B.; Strano, Michael S.

doi:10.1002/smll.201802086

Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Journal Article · Thu Sep 06 00:00:00 EDT 2018 · Small

DOI:https://doi.org/10.1002/smll.201802086· OSTI ID:1609981

Lew, Tedrick Thomas Salim ^[1]; Wong, Min Hao ^[2]; Kwak, Seon-Yeong ^[2]; Sinclair, Rosalie ^[2]; Koman, Volodymyr B. ^[2]; Strano, Michael S. ^[2]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); DOE/OSTI
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

The ability to control the subcellular localization of nanoparticles within living plants offers unique advantages for targeted biomolecule delivery and enables important applications in plant bioengineering. However, the mechanism of nanoparticle transport past plant biological membranes is poorly understood. Here, a mechanistic study of nanoparticle cellular uptake into plant protoplasts is presented. An experimentally validated mathematical model of lipid exchange envelope penetration mechanism for protoplasts, which predicts that the subcellular distribution of nanoparticles in plant cells is dictated by the particle size and the magnitude of the zeta potential, is advanced. The mechanism is completely generic, describing nanoparticles ranging from quantum dots, gold and silica nanoparticles, nanoceria, and single-walled carbon nanotubes (SWNTs). In addition, the use of imaging flow cytometry to investigate the influence of protoplasts' morphological characteristics on nanoparticle uptake efficiency is demonstrated. Using DNA-wrapped SWNTs as model nanoparticles, it is found that glycerolipids, the predominant lipids in chloroplast membranes, exhibit stronger lipid–nanoparticle interaction than phospholipids, the major constituent in protoplast membrane. This work can guide the rational design of nanoparticles for targeted delivery into specific compartments within plant cells without the use of chemical or mechanical aid, potentially enabling various plant engineering applications.

View Accepted Manuscript (DOE)

Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-08ER46488

OSTI ID:: 1609981

Alternate ID(s):: OSTI ID: 1469026

Journal Information:: Small, Journal Name: Small Journal Issue: 44 Vol. 14; ISSN 1613-6810

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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